Isolation and characterization of collagen from brown backed toadfish ( Lagocephalus gloveri) skin

• Published in: Bioresource technology 2006, Vol.97 (2), p.191-197
• Main Authors: Senaratne, L.S., Park, Pyo-Jam, Kim, Se-Kwon
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Amino Acids - analysis; Animals; Batrachoidiformes; Biological and medical sciences; Brown backed toadfish; Characterization; Collagen; Collagen - chemistry; Collagen - isolation & purification; fish products; fish waste; Food industries; Fundamental and applied biological sciences. Psychology; General agronomy. Plant production; Isolation; Lagocephalus gloveri; marine fish; Peptide Mapping; Processing waste; Protein Denaturation; skin; Skin - chemistry; Temperature; Tetradonematidae; Use and upgrading of agricultural and food by-products. Biotechnology; Use of agricultural and forest wastes. Biomass use, bioconversion; waste utilization
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2005.02.024

Collagen was isolated and characterized from the skin of brown backed toadfish ( Lagocephalus gloveri), processing wastes. The total collagen yield extracted was 54.3% on the basis of lyophilized dry weight compared to other vertebrates. According to the electrophoretic pattern, the skin collagen of the fish consisted of heterotrimer α 1 α 2 α 3. Moreover, the denaturation temperature was 28 °C, which was about 9 °C lower than that of collagen from porcine skin. Therefore, there is a possibility to use brown backed toadfish skin collagen as an alternative source of collagen for industrial purposes and subsequently it may maximize the economical value of the fish.