Antiplatelet and antithrombotic activity of indole-3-carbinol in vitro and in vivo

Indole-3-carbinol, a natural compound found in cruciferous vegetables, is known to have anticancer activity. In the present study, the antiplatelet and antithrombotic activities of indole-3-carbinol were investigated in vitro and in vivo. Indole-3-carbinol significantly inhibited collagen-induced pl...

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Published in:Phytotherapy research 2008-01, Vol.22 (1), p.58-64
Main Authors: Park, Min-Kyu, Rhee, Yun-Hee, Lee, Hyo-Jung, Lee, Eun-Ok, Kim, Kwan-Hyun, Park, Min-Jong, Jeon, Byung-Hun, Shim, Bum-Sang, Jung, Chang-Hyun, Choi, Seung-Hoon, Ahn, Kyoo-Seok, Kim, Sung-Hoon
Format: Article
Language:English
Subjects:
Animals
antiplatelet activity
antithrombotic agent
Biological and medical sciences
Collagen - pharmacology
Dinoprostone - metabolism
Dose-Response Relationship, Drug
Epinephrine - metabolism
Fibrinogen - metabolism
Fibrinolytic Agents - chemistry
Fibrinolytic Agents - pharmacology
Flow Cytometry
General pharmacology
glycoprotein IIb/IIIa
Humans
indole-3-carbinol
Indoles - chemistry
Indoles - pharmacology
Medical sciences
Mice
Molecular Structure
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Platelet Aggregation - drug effects
Platelet Aggregation Inhibitors - chemistry
Platelet Aggregation Inhibitors - pharmacology
Platelet Glycoprotein GPIIb-IIIa Complex - metabolism
Protein Binding - drug effects
thromboxane B2
Thromboxane B2 - metabolism
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Summary:Indole-3-carbinol, a natural compound found in cruciferous vegetables, is known to have anticancer activity. In the present study, the antiplatelet and antithrombotic activities of indole-3-carbinol were investigated in vitro and in vivo. Indole-3-carbinol significantly inhibited collagen-induced platelet aggregation in human platelet rich plasma (PRP) in a concentration-dependent manner. Indole-3-carbinol significantly inhibited fibrinogen binding to the platelet surface glycoprotein IIb/IIIa (GP IIb/IIIa) receptor by flow cytometric analysis. In addition, the levels of thromboxane B₂ (TXB₂) and prostaglandin E₂ (PGE₂) in collagen stimulated PRP were significantly inhibited in a concentration-dependent manner by indole-3-carbinol. Furthermore, indole-3-carbinol dose-dependently suppressed the death of mice with pulmonary thrombosis induced by intravenous injection of collagen and epinephrine. These results suggest that indole-3-carbinol can be a potent antithrombotic agent with antiplatelet activity through the inhibition of GP IIb/IIIa receptor and thromboxane B₂ formation. Copyright © 2007 John Wiley & Sons, Ltd.
ISSN:0951-418X
1099-1573
DOI:10.1002/ptr.2260