Visualisation of bacterial sequestration and bactericidal activity within hydrating Hydrofiber ® wound dressings

• Published in: Biomaterials 2006-03, Vol.27 (7), p.1129-1139
• Main Authors: Newman, Geoffrey R., Walker, Michael, Hobot, Jan A., Bowler, Philip G.
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - administration & dosage; Bacteria; Bandages, Hydrocolloid - microbiology; Biofilms - drug effects; Confocal microscopy; Drug Delivery Systems - methods; Equipment Failure Analysis - methods; Hydrofiber ® wound dressing; Materials Testing - methods; Microscopy, Confocal - methods; Microscopy, Fluorescence - methods; Pseudomonas aeruginosa - cytology; Pseudomonas aeruginosa - drug effects; Silver; Silver - administration & dosage; Silver - chemistry; Staphylococcus aureus - cytology; Staphylococcus aureus - drug effects; Water - chemistry; Wound healing
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/j.biomaterials.2005.07.046

The fluid handling and microbiological properties of a non-antimicrobial Hydrofiber ® (NAH) wound dressing have been compared with those of a silver salt-containing Hydrofiber ® (SCH). Fluorescent dyes ( BacLight™, Live/Dead™ Kit) were added to fresh cultures of two wound pathogens ( Pseudomonas aeruginosa and Staphylococcus aureus), and used to visualise their viability. Live bacteria stained green and dead/dying bacteria turned red. When inoculated into samples of the NAH and SCH dressings, the viability of the bacteria could be effectively monitored over time using a rapid form of confocal laser scanning microscopy (RCLSM—Leica ® UK). When the NAH dressing was hydrated with stained bacterial culture, its fibres swelled quickly, reducing interstitial spaces between the fibres, resulting in the formation of a cohesive gel. Bacteria became immobilised in the gel, forming characteristic clumps, but remained largely green (viable) for more than 20 h with no apparent increase in numbers. The SCH initially behaved in a similar manner, however, using 3-D data from RCLSM time-lapse sequences P. aeruginosa was observed to turn progressively red (i.e. died) within 1.5–3 h and S. aureus similarly turned red within 5–7 h of contact with the SCH dressing. The ability of both Hydrofiber ® dressings to sequester and immobilise potentially pathogenic wound micro-organisms has been demonstrated. Additionally the SCH dressing was shown to kill immobilised bacteria, as a consequence of the ionic silver bactericide. These properties of the Hydrofiber ® dressings may contribute to providing an environment that is supportive to wound healing.