Tone Reversal of an AFM Lateral Force Image Due to Hybridization of Oligonucleotides Immobilized on Polymers

The interaction between oligonucleotide strands and polymer chains on surfaces has been investigated by AFM and XPS, with cyclo‐olefin‐copolymer (COC) and polycarbonate (PC) as model polymers (see image). Lower friction recorded by the AFM tip when mapping the aggregates of covalently bound amino‐te...

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Bibliographic Details
Published in:Small (Weinheim an der Bergstrasse, Germany) Germany), 2005-06, Vol.1 (6), p.610-613
Main Authors: Nicolau, Dan V., Pham, Duy K., Ivanova, Elena P., Wright, Jonathan P., Lenigk, Ralf, Smekal, Thomas, Grodzinski, Piotr
Format: Article
Language:English
Subjects:
AFM
Alkenes - chemistry
DNA - chemistry
DNA immobilization
DNA recognition
DNA, Single-Stranded - chemistry
lateral force analysis
Microscopy, Atomic Force - methods
Nanotechnology - instrumentation
Nanotechnology - methods
Nucleic Acid Hybridization
Oligonucleotides - chemistry
Polycarboxylate Cement - chemistry
Polymers - chemistry
Surface Properties
surfaces
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Summary:The interaction between oligonucleotide strands and polymer chains on surfaces has been investigated by AFM and XPS, with cyclo‐olefin‐copolymer (COC) and polycarbonate (PC) as model polymers (see image). Lower friction recorded by the AFM tip when mapping the aggregates of covalently bound amino‐terminated ssDNA chains on COC and PC surfaces can be explained by the preferential orientation of the free hydrophobic bases towards the outside of the aggregates; hybridization of the ssDNA aggregates leads to tone reversal of the lateral force image.
ISSN:1613-6810
1613-6829
DOI:10.1002/smll.200400125