Responsiveness of intestinal epithelial cell turnover to TGF-α after bowel resection in a rat is correlated with EGF receptor expression along the villus–crypt axis

Recent evidence suggests that transforming growth factor alpha (TGF-α) enhances enterocyte proliferation and stimulates intestinal adaptation after massive bowel resection. In the present study, we evaluated the effects of TGF-α on enterocyte turnover and correlated it with epidermal-growth factor (...

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Bibliographic Details
Published in:Pediatric surgery international 2008, Vol.24 (1), p.21-28
Main Authors: Sukhotnik, Igor, Mogilner, Jorge G., Shaoul, Ron, Karry, Rahel, Lieber, Michael, Suss-Toby, Edith, Ure, Benno M., Coran, Arnold G.
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Caspase 3 - metabolism
Cell Proliferation - drug effects
Disease Models, Animal
Enterocytes - drug effects
Enterocytes - metabolism
Enterocytes - pathology
Follow-Up Studies
Gene Expression
Immunohistochemistry
Male
Medicine
Medicine & Public Health
Microscopy, Confocal
Original Article
Pediatric Surgery
Pediatrics
Rats
Rats, Sprague-Dawley
Receptor, Epidermal Growth Factor - biosynthesis
Receptor, Epidermal Growth Factor - genetics
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
Short Bowel Syndrome - drug therapy
Short Bowel Syndrome - metabolism
Short Bowel Syndrome - pathology
Surgery
Transforming Growth Factor alpha - therapeutic use
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Summary:Recent evidence suggests that transforming growth factor alpha (TGF-α) enhances enterocyte proliferation and stimulates intestinal adaptation after massive bowel resection. In the present study, we evaluated the effects of TGF-α on enterocyte turnover and correlated it with epidermal-growth factor (EGF) receptor expression along the villus–crypt axis in a rat model of short bowel syndrome (SBS). Male rats were divided into three groups, sham rats underwent bowel transection (group A); SBS rats underwent a 75% bowel resection (group B); and SBS/TGF-α rats underwent bowel resection and were treated with TGF-α (75 μg/kg) (group C) from the seventh postoperative day. Parameters of intestinal adaptation, enterocyte proliferation and apoptosis were determined on day 15. Villus tips, lateral villi and crypts were separated using laser capture microdissection. EGF receptor expression for each compartment was assessed by quantitative real-time PCR (Taqman). Statistical analysis was performed using one-way ANOVA test, with P  
ISSN:0179-0358
1437-9813
DOI:10.1007/s00383-007-2038-z