Production and properties of a surfactant obtained from Bacillus subtilis grown on cassava wastewater

The production and properties of a biosurfactant, synthesized by Bacillus subtilis LB5a strain, using cassava wastewater as substrate were investigated. The microorganism was able to grow and to produce surfactant on cassava waste, reducing the surface tension of medium to 26.6 mN/m and giving a cru...

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Bibliographic Details
Published in:Bioresource technology 2006, Vol.97 (2), p.336-341
Main Authors: Nitschke, Marcia, Pastore, Glaucia Maria
Format: Article
Language:English
Subjects:
Accumulation
Bacillus subtilis
Bacillus subtilis - chemistry
Bacillus subtilis - metabolism
Biological and medical sciences
Biosurfactant
biosynthesis
Biotechnology
cassava
Cassava wastewater
cell culture
emulsifying properties
Food industries
food processing wastes
Fundamental and applied biological sciences. Psychology
Hot Temperature
Hydrogen-Ion Concentration
Lipopeptide
lipopeptides
Manihot - chemistry
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Surface-Active Agents - chemistry
Surface-Active Agents - isolation & purification
Surface-Active Agents - metabolism
surfactants
Time Factors
Use and upgrading of agricultural and food by-products. Biotechnology
Waste Disposal, Fluid - methods
wastewater
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Summary:The production and properties of a biosurfactant, synthesized by Bacillus subtilis LB5a strain, using cassava wastewater as substrate were investigated. The microorganism was able to grow and to produce surfactant on cassava waste, reducing the surface tension of medium to 26.6 mN/m and giving a crude surfactant concentration of 3.0 g/L after 48 h. The surface-active compound retained its properties during exposure to elevate temperatures (100 °C), high salinity (20% NaCl) and a wide range of pH values. The surfactant was capable of forming stable emulsions with various hydrocarbons. Preliminary chemical characterization revealed that the surfactant has a lipopeptide composition with a CMC value of about 33 mg/L. Cassava wastewater proved to be a suitable substrate for biosurfactant biosynthesis, providing not only bacterial growth and product accumulation but also a surfactant that has interesting and useful properties with potential for many industrial applications.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2005.02.044