Biases in human papillomavirus genotype prevalence assessment associated with commonly used consensus primers

Consensus primers targeting human papillomaviruses (HPVs) have biases in sensitivity toward certain HPV types. We applied 3 primer sets (GP5+/6+, MY09/11, PGMY09/11) in parallel on 120 Chinese cervical cancer specimens. GP5+/6+ exhibited a poor sensitivity for HPV52, for which the prevalence among s...

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Bibliographic Details
Published in:International journal of cancer 2006, Vol.118 (1), p.243-245
Main Authors: CHAN, Paul K. S, CHEUNG, Tak-Hong, MAMIE HUI, IP, Margaret, TAM, Ann O. Y, LO, Keith W. K, YIM, So-Fan, YU, May M. Y, TO, Ka-Fai, WONG, Yick-Fu, CHEUNG, Jo L. K, CHAN, Denise P. C
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cancer Vaccines
Carcinoma, Squamous Cell - virology
DNA Primers
Female
Female genital diseases
Genotype
Gynecology. Andrology. Obstetrics
Humans
Medical sciences
Papillomaviridae - genetics
Papillomaviridae - pathogenicity
Papillomavirus Infections - diagnosis
Papillomavirus Infections - epidemiology
Papillomavirus Infections - genetics
Prevalence
Reproducibility of Results
Sensitivity and Specificity
Tropical medicine
Tumors
Uterine Cervical Neoplasms - virology
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Summary:Consensus primers targeting human papillomaviruses (HPVs) have biases in sensitivity toward certain HPV types. We applied 3 primer sets (GP5+/6+, MY09/11, PGMY09/11) in parallel on 120 Chinese cervical cancer specimens. GP5+/6+ exhibited a poor sensitivity for HPV52, for which the prevalence among squamous cell cervical cancer was underestimated from 14.6% to 0%. The fact that HPV52 should rank second in prevalence among squamous cell cervical carcinoma in Hong Kong could be missed if GP5+/6+, a worldwide commonly used primer set, was selected for HPV detection. Biases in HPV type-specific sensitivity may result in misprioritization of vaccine candidates.
ISSN:0020-7136
1097-0215
DOI:10.1002/ijc.21299