Relationship between plasma levels of soluble CD40L and insulin sensitivity and insulin secretion status in non-diabetic dyslipidemic patients

• Published in: Diabetes research and clinical practice 2008-01, Vol.79 (1), p.48-55
• Main Authors: Filozof, Claudia, Gómez-Garre, Dulcenombre, Reinares, Leonardo, González-Rubio, María Luisa, Muñoz-Pacheco, Paloma, Rueda, Antonio, Álvarez-Arcaya, Arántzazu, Calle-Pascual, Alfonso L, Fernández-Cruz, Arturo
• Format: Article
• Language: English
• Subjects: Adult; Blood Platelets - physiology; Blood Pressure; Body Size; Body Weight; CD40 Ligand - blood; CD40L; Dyslipidemias - blood; Dyslipidemias - drug therapy; Dyslipidemias - immunology; Dyslipidemic patients; Endocrinology & Metabolism; Female; Flow Cytometry; Homeostasis model assessment (HOMA); Humans; Hydroxymethylglutaryl-CoA Reductase Inhibitors - therapeutic use; Insulin - blood; Insulin - metabolism; Insulin Resistance - physiology; Insulin Secretion; Male; Middle Aged
• ISSN: 0168-8227; 1872-8227
• DOI: 10.1016/j.diabres.2007.07.002

Abstract Objective We have measured circulating plasma sCD40L as well as the platelet-surface of CD40L and its receptor in a sample of non-diabetic dyslipidemic patients and then evaluated its relationship with the insulin resistance (IR) and insulin secretion (IS) status. Design and methods Anthropometric measurements, fasting glucose, insulin, lipids, and IR and IS [estimated by the homeostasis model assessment (HOMA)] were assessed in 86 dyslipidemic subjects. Circulating sCD40L were determined by ELISA. By flow cytometry, CD40L, CD40 and P-selectin were evaluated in the platelet-surface. Results Non-diabetic dyslipidemic IR patients (HOMA-IR ≥ 3.8) showed higher plasma sCD40L concentrations and a more unfavorable cardiovascular risk profile (higher BMI, waist, fasting insulin and mean triglyceride levels) than dyslipidemic patients with low IS (HOMA β-cell < 98). In a multivariable model, only measures of insulin sensitivity and higher waist remained significantly associated with increased plasma levels of sCD40L. Surface expression of CD40L on platelets decreased significantly and CD40 increased in IR patients, compared with patients with low IS. Conclusions IR dyslipidemic patients show increased plasma sCD40L and decreased platelet-membrane CD40L expression compared to dyslipidemic patients with low IS.