Rectifying system-specific errors in NMR relaxation measurements

15N spin relaxation parameters provide a powerful tool for probing the internal dynamics and thermodynamics of proteins. The biological insight provided by these experiments often involves interpretation of small changes in relaxation parameters. This, in turn, requires careful data analysis, especi...

Full description

Saved in:
Bibliographic Details
Published in:Journal of magnetic resonance (1997) 2006, Vol.178 (1), p.77-87
Main Authors: Pawley, Norma H., Clark, M. Daniel, Michalczyk, Ryszard
Format: Article
Language:English
Subjects:
NMR
Nuclear Magnetic Resonance, Biomolecular - methods
Protein dynamics
Relaxation dispersion
Signal Processing, Computer-Assisted
Systematic error
Temperature
Ubiquitin - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:15N spin relaxation parameters provide a powerful tool for probing the internal dynamics and thermodynamics of proteins. The biological insight provided by these experiments often involves interpretation of small changes in relaxation parameters. This, in turn, requires careful data analysis, especially in the identification and treatment of systematic error. While progress continues on reduction of experiment-specific errors associated with pulse sequences, system-specific sources of error have received far less attention. The impact of these errors varies between facilities, spectrometers, and biological samples. We demonstrate that performing a series of control experiments along with relaxation measurements can help identify, quantify, and isolate sources of system-specific error, and, in some cases, correct for systematic changes. We further demonstrate that control experiments can be performed without significant loss of spectrometer time, and lead to more accurate relaxation parameter values.
ISSN:1090-7807
1096-0856
DOI:10.1016/j.jmr.2005.08.017