Impact of forskolin and amino acid depletion upon System A activity and SNAT expression in BeWo cells

Amino acid transport System A (SysA) plays an important role in mediating the transplacental transfer of neutral amino acids from mother to fetus. Given that prior work has demonstrated that SysA activity is regulated, both over gestation and in response to dietary restriction during pregnancy, we e...

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Bibliographic Details
Published in:Biochimie 2006, Vol.88 (1), p.39-44
Main Authors: Novak, Don, Quiggle, Forest, Haafiz, Allah
Format: Article
Language:English
Subjects:
Amino acid transport
Amino Acid Transport System A - biosynthesis
Amino Acid Transport System A - drug effects
Amino Acid Transport System A - metabolism
Amino Acids - deficiency
beta-Alanine - analogs & derivatives
beta-Alanine - metabolism
BeWo cells
Cells, Cultured
Colforsin - pharmacology
Female
Humans
Placenta
Pregnancy
SNAT
System A
Up-Regulation
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Summary:Amino acid transport System A (SysA) plays an important role in mediating the transplacental transfer of neutral amino acids from mother to fetus. Given that prior work has demonstrated that SysA activity is regulated, both over gestation and in response to dietary restriction during pregnancy, we examined the response of SysA activity and sodium-dependent neutral amino acid transporter (SNAT; responsible for SysA activity) expression to cAMP analogues and amino acid deprivation in BeWo cells, an accepted model of placental syncytia. SysA activity was unaffected by forskolin, a cAMP agonist, at 48 and 72 h. Amino acid depletion was associated with an up-regulation of SysA activity, largely mediated through an enhancement of SNAT2 (Slc38a2) expression at both the protein and mRNA level. SNAT1 (Slc38a1) expression did not change in response to amino acid depletion, while SNAT4 (Slc38a4) could not be detected. In summary, SysA activity in BeWo cells responds to amino acid depletion through the differential regulation of SNAT subtypes.
ISSN:0300-9084
1638-6183
DOI:10.1016/j.biochi.2005.07.002