Protective and cross-protective mucosal immunization of mice by influenza virus type A with bacterial adjuvant

Abstract Mucosal immunization by inactivated viruses often fails to evoke a sufficient immune response. Intensive efforts have been made to enhance the response by suitable adjuvants. We used the G+ nonpathogenic delipidated bacterium Bacillus firmus with pronounced immunostimulatory properties as a...

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Bibliographic Details
Published in:Immunology letters 2008-01, Vol.115 (2), p.144-152
Main Authors: Zanvit, Peter, Havlíčková, Martina, Táčner, Jaroslav, Novotná, Olga, Jirkovská, Marie, Čechová, Dana, Julák, Jaroslav, Šterzl, Ivan, Prokešová, Ludmila
Format: Article
Language:English
Subjects:
Adjuvant immunization
Adjuvants, Immunologic
Allergy and Immunology
Animals
Antibodies, Viral - blood
Antibodies, Viral - immunology
Bacillus - immunology
Bacillus firmus
Cross Reactions
Cross-protection
Cytokines - biosynthesis
Cytokines - immunology
Female
Humans
Immunity, Mucosal
Immunization
Influenza A virus
Influenza A Virus, H1N1 Subtype - immunology
Influenza A Virus, H3N2 Subtype - immunology
Influenza Vaccines - immunology
Influenza virus
Influenza virus type A
Influenza, Human - immunology
Influenza, Human - mortality
Influenza, Human - prevention & control
Influenza, Human - virology
Lung - immunology
Lung - pathology
Mice
Mice, Inbred BALB C
Orthomyxoviridae Infections - immunology
Orthomyxoviridae Infections - mortality
Orthomyxoviridae Infections - prevention & control
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Summary:Abstract Mucosal immunization by inactivated viruses often fails to evoke a sufficient immune response. Intensive efforts have been made to enhance the response by suitable adjuvants. We used the G+ nonpathogenic delipidated bacterium Bacillus firmus with pronounced immunostimulatory properties as an adjuvant for immunizing mice with inactivated influenza virus type A. BALB/c mice were immunized intratracheally with inactivated influenza A H1N1 and H3N2 viruses. The production of antibodies in sera and secretions was determined by the ELISA. The local situation in the lungs was assessed histologically and by testing the cytokine expression. The protective and cross-protective effect against infection was tested in in vivo experiments after infection with influenza virus A H1N1. B. firmus as adjuvant increased both systemic and mucosal antibody responses, improved protection against homologous virus and induced cross-protection against virus H1N1 after immunization with virus H3N2.
ISSN:0165-2478
1879-0542
DOI:10.1016/j.imlet.2007.11.001