Evidence for a sorting endosome in Arabidopsis root cells

In eukaryotic cells, the endocytic and secretory pathways are key players in several physiological processes. These pathways are largely inter-connected in animal and yeast cells through organelles named sorting endosomes. Sorting endosomes are multi-vesicular compartments that redirect proteins tow...

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Bibliographic Details
Published in:The Plant journal : for cell and molecular biology 2008-01, Vol.53 (2), p.237-247
Main Authors: Jaillais, Yvon, Fobis-Loisy, Isabelle, Miège, Christine, Gaude, Thierry
Format: Article
Language:English
Subjects:
Arabidopsis
Arabidopsis - physiology
Arabidopsis Proteins - metabolism
Biological and medical sciences
Botany
Cell kinetics
Cell Membrane - metabolism
Cell physiology
Cellular biology
Cytoplasmic Vesicles - physiology
Endocytosis
Endosomes - physiology
Fundamental and applied biological sciences. Psychology
Membrane Proteins - metabolism
Membranes
Plant physiology and development
Plant Roots - physiology
Plant Roots - ultrastructure
pre-vacuolar compartment
protein trafficking
protein transport
Protein Transport - physiology
Proteins
root cells
sorting endosome
sorting nexin
trans-Golgi Network - physiology
Vesicular Transport Proteins - metabolism
Yeast
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Summary:In eukaryotic cells, the endocytic and secretory pathways are key players in several physiological processes. These pathways are largely inter-connected in animal and yeast cells through organelles named sorting endosomes. Sorting endosomes are multi-vesicular compartments that redirect proteins towards various destinations, such as the lysosomes or vacuoles for degradation, the trans-Golgi network for retrograde transport and the plasma membrane for recycling. In contrast, cross-talk between the endocytic and secretory pathways has not been clearly established in plants, especially in terms of cargo protein trafficking. Here we show by co-localization analyses that endosomes labelled with the AtSORTING NEXIN1 (AtSNX1) protein overlap with the pre-vacuolar compartment in Arabidopsis root cells. In addition, alteration of the routing functions of AtSNX1 endosomes by drug treatments leads to mis-routing of endocytic and secretory cargo proteins. Based on these results, we propose that the AtSNX1 endosomal compartment represents a sorting endosome in root cells, and that this specialized organelle is conserved throughout eukaryotes.
ISSN:0960-7412
1365-313X
DOI:10.1111/j.1365-313X.2007.03338.x