Antimicrobial activity of long-chain, water-soluble, dendritic tricarboxylato amphiphiles

• Published in: Journal of antimicrobial chemotherapy 2007-03, Vol.59 (3), p.451-458
• Main Authors: Williams, André A., Sugandhi, Eko W., Macri, Richard V., Falkinham, Joseph O., Gandour, Richard D.
• Format: Article
• Language: English
• Subjects: Anti-Infective Agents - pharmacology; Antibiotics. Antiinfectious agents. Antiparasitic agents; Aspergillus niger; Bacteria; Bacteria - drug effects; Biological and medical sciences; Candida albicans; Chemical synthesis; Cryptococcus neoformans; Escherichia coli; Fatty acids; Fatty Acids - chemical synthesis; Fatty Acids - pharmacology; Fungi; Fungi - drug effects; Klebsiella pneumoniae; Lactobacillus plantarum; Medical research; Medical sciences; Microbial Sensitivity Tests; Microbiology; Micrococcus luteus; mycobacteria; Mycobacterium smegmatis; Pharmacology. Drug treatments; Saccharomyces cerevisiae; Solubility; Staphylococcus aureus; Structure-Activity Relationship; synthetic lipids
• ISSN: 0305-7453; 1460-2091
• DOI: 10.1093/jac/dkl503

Objectives To measure the antimicrobial activities of three series of homologous, dendritic tricarboxylato (three-headed) amphiphiles against a battery of bacteria and fungi. Methods Three series of homologous dendritic amphiphiles were synthesized containing C13 to C22 fatty chains. Susceptibility of Escherichia coli, Klebsiella pneumoniae, Lactobacillus plantarum, Micrococcus luteus, Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), Mycobacterium smegmatis, Saccharomyces cerevisiae, Candida albicans, Cryptococcus neoformans and Aspergillus niger to the amphiphiles was measured by broth microdilution and reported as the MIC. Results Several amphiphiles from each homologous series, designed and constructed to overcome the low solubility of saturated long-chain fatty acids, had antimicrobial activity against MRSA (MIC = 36 mg/L), C. albicans (MIC = 4.4 mg/L), S. cerevisiae (MIC = 1.1 mg/L) and M. smegmatis (MIC = 8.9 mg/L). These amphiphiles had considerably better antimicrobial activities than the corresponding saturated fatty acids. Alkyl chain length influenced the values of MIC; longer chains (C18–C22) were generally more antimicrobial, but there was no uniform pattern among the microorganisms tested. Conclusions As the antimicrobial activity of the amphiphiles increased with increasing chain length, it is anticipated that maximum activity was not reached with these series. Thus, the identification of the optimal chain length would provide a target compound for development of low-cost, topical microbicides and anti-infectives. Further, these series of dendritic amphiphiles with the very long chains can be used as new water-soluble probes for elucidation of membrane structure and for identification of novel targets for antimicrobial design.