Isolation, structural elucidation and characterization of impurities in Cefdinir

Three unknown impurities in Cefdinir bulk drug at levels below 0.2% (ranging from 0.05 to 0.2%) have been detected by high performance liquid chromatography (HPLC). These impurities were isolated from crude sample of Cefdinir using preparative HPLC. Based on the spectral data (NMR, IR and MS) the st...

Full description

Saved in:
Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2007-03, Vol.43 (4), p.1476-1482
Main Authors: Rao, K.V.V. Prasada, Rani, A., Reddy, A.V. Raghava, Bharathi, C.H., Dandala, Ramesh, Naidu, A.
Format: Article
Language:English
Subjects:
Analysis
Analytical, structural and metabolic biochemistry
Anti-Bacterial Agents - analysis
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - standards
Biological and medical sciences
Cefdinir
Cephalosporins - analysis
Cephalosporins - chemistry
Cephalosporins - standards
Characterization
Chromatography, High Pressure Liquid
Drug Contamination
Fundamental and applied biological sciences. Psychology
General pharmacology
Identification
Impurities
Magnetic Resonance Spectroscopy
Mass Spectrometry
Medical sciences
Molecular Structure
Pharmacology. Drug treatments
Preparative HPLC
Spectroscopy
Spectroscopy, Fourier Transform Infrared
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Three unknown impurities in Cefdinir bulk drug at levels below 0.2% (ranging from 0.05 to 0.2%) have been detected by high performance liquid chromatography (HPLC). These impurities were isolated from crude sample of Cefdinir using preparative HPLC. Based on the spectral data (NMR, IR and MS) the structures of these impurities were characterized as (6R, 7R)-7-[(z)-2-(2-aminothiazol-4-yl)-2-hydroxyiminoacetamido]-8-oxo-3-vinyl-5-thia-1-azabicyclo [4.2.0] oct-2-ene-2-carboxylic acid-5-oxide (I). (6R, 7R)-7-[(z)-2-(2-aminothiazol-4-yl)-2-hydroxyiminoacetamido]-8-oxo-3-vinyl-5-thia-1-azabi-cyclo [4.2.0] oct-3-ene-2-carboxylic acid (II). (6R, 7R)-7-[(z)-2-(2-aminothiazol-4-yl)-2-hydroxyiminoacetamido]-8-oxo-3-methyl-5-thia-1-azabicyclo-[4.2.0]oct-2-ene-2-carboxylic acid (III), respectively. The origin and structural elucidation of all impurities have been discussed.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2006.10.031