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IDI2, a Second Isopentenyl Diphosphate Isomerase in Mammals

We recently described the identification of a novel isopentenyl diphosphate isomerase, IDI2 in humans and mice. Our current data indicate that, in humans, IDI2 is expressed only in skeletal muscle. Expression constructs of human IDI2 in Saccharomyces cerevisiae can complement isomerase function in a...

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Published in:	The Journal of biological chemistry 2007-03, Vol.282 (9), p.6668-6676
Main Authors:	Clizbe, Daun B., Owens, Michelle L., Masuda, Kimberly R., Shackelford, Janis E., Krisans, Skaidrite K.
Format:	Article
Language:	English
Subjects:	Animals Carbon-Carbon Double Bond Isomerases - genetics Carbon-Carbon Double Bond Isomerases - metabolism Catalysis Cloning, Molecular Genetic Complementation Test Hemiterpenes Humans Isoenzymes Isomerism Kinetics Mice Peroxisomes - enzymology PPAR alpha Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics
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container_title	The Journal of biological chemistry
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creator	Clizbe, Daun B. Owens, Michelle L. Masuda, Kimberly R. Shackelford, Janis E. Krisans, Skaidrite K.
description	We recently described the identification of a novel isopentenyl diphosphate isomerase, IDI2 in humans and mice. Our current data indicate that, in humans, IDI2 is expressed only in skeletal muscle. Expression constructs of human IDI2 in Saccharomyces cerevisiae can complement isomerase function in an idi1-deficient yeast strain. Furthermore, IDI2 has the ability to catalyze the isomerization of [14C]IPP to [14C]DMAPP. Enzyme kinetic analysis of partially purified IDI2 demonstrate the novel isozyme has a maximal relative specific activity of 1.2 × 10-1 ± 0.3 μmol min-1 mg-1 at pH 8.0 with a KIPPm value of 22.8 μm IPP. Both isozymes, IDI1 and IDI2 are localized to the peroxisome by a PTS1-dependent pathway. Finally, our data suggest that IDI2 is regulated independently from IDI1, by a mechanism that may involve PPARα.
doi_str_mv	10.1074/jbc.M610922200
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subjects	Animals Carbon-Carbon Double Bond Isomerases - genetics Carbon-Carbon Double Bond Isomerases - metabolism Catalysis Cloning, Molecular Genetic Complementation Test Hemiterpenes Humans Isoenzymes Isomerism Kinetics Mice Peroxisomes - enzymology PPAR alpha Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics
title	IDI2, a Second Isopentenyl Diphosphate Isomerase in Mammals
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