YOYO as a Dye to Track Penetration of LK15 DNA Complexes in Spheroids: Use and Limits

• Published in: Journal of fluorescence 2008-01, Vol.18 (1), p.155-161
• Main Authors: Perry, Heather AD, Alhaj Saleh, Amer F., Aojula, Harmesh, Pluen, Alain
• Format: Article
• Language: English
• Subjects: Analytical Chemistry; Benzoxazoles - chemistry; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biomedicine; Biophysics; Biotechnology; DNA - chemistry; DNA - metabolism; Fluorescent Dyes - chemistry; HCT116 Cells; Humans; Hyaluronoglucosaminidase - metabolism; Luciferases - metabolism; Original Paper; Peptide Fragments - chemistry; Peptide Fragments - metabolism; Plasmids; Quinolinium Compounds - chemistry; Spectrometry, Fluorescence; Spheroids, Cellular - metabolism
• ISSN: 1053-0509; 1573-4994
• DOI: 10.1007/s10895-007-0254-5

To elucidate the reasons underlying the poor penetration of non-viral vectors in tissues, relating transport properties to physico-chemical parameters of vectors may be crucial. These properties can be influenced by the presence of multiples labels that are used. Therefore utilizing a vector with minimum of labels preferably not more than one is important to studying penetration in tissues. The cell impermeant bisintercalating dye YOYO-1 was found suitable to both monitor the formation of complexes between DNA and an amphipathic peptide LK15 and, to track their penetration in HCT116 spheroids by confocal microscopy. The results revealed a limited decrease of fluorescence ascribed to the high affinity of YOYO-1 to bind DNA. The residual fluorescence of complexes can be exploited to monitor penetration into spheroids, after correction for YOYO-1 attenuation, and to revealing hyaluronidase-induced reduced binding. Hence high affinity dyes such as YOYO-1 with inefficiently quenched fluorescence may be important to establish a relation between novel medicines characteristics and penetration in tissues.