Reversed-phase liquid chromatography column testing and classification: Physicochemical interpretation based on a wide set of stationary phases

The high number of stationary phases commercially available for liquid chromatography makes the choice of the analyst a real headache. In order to provide a tool to carry out this choice on objective basis, the present work proposes interpretations of the column classifications obtained, thanks to a...

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Bibliographic Details
Published in:Journal of Chromatography A 2007-03, Vol.1144 (2), p.183-196
Main Authors: Le Mapihan, K., Vial, J., Jardy, A.
Format: Article
Language:English
Subjects:
Analysis
Analytical chemistry
Basic compounds
Biological and medical sciences
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, Liquid - classification
Chromatography, Liquid - instrumentation
Chromatography, Liquid - methods
Cluster Analysis
Column characterization
Exact sciences and technology
General pharmacology
Medical sciences
Organic Chemicals - chemistry
Other chromatographic methods
Pharmaceuticals
Pharmacology. Drug treatments
Principal component analysis
Research Design
Sensitivity and Specificity
Solvent effect
Solvents - chemistry
Spectrophotometry, Ultraviolet - methods
Stationary phases
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Summary:The high number of stationary phases commercially available for liquid chromatography makes the choice of the analyst a real headache. In order to provide a tool to carry out this choice on objective basis, the present work proposes interpretations of the column classifications obtained, thanks to a previously described testing procedure. The meaning of principal components was attributed to crossing over information carried by loading plots and groups revealed by hierarchical cluster analysis (HCA) on the corresponding score plots. At high solvent ratio, the retention seemed to be governed by enthalpy, whereas at low solvent ratio, entropic phenomena were predominating. Finally, the behavior of known families of RPLC columns was studied giving rise either to homogeneous groups like polar embedded grafts columns or to scattered families like Aqua type columns.
ISSN:0021-9673
DOI:10.1016/j.chroma.2007.01.034