Cryopreservation of porcine articular cartilage: MRI and biochemical results after different freezing protocols

The objective of this study was to investigate the effects of cryopreservation on the components of articular cartilage (AC) matrix by utilizing magnetic resonance imaging (MRI) and biochemical assessments. Porcine AC (10 mm osteochondral dowels) was collected into four groups – (1) phosphate buffer...

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Bibliographic Details
Published in:Cryobiology 2007-02, Vol.54 (1), p.36-43
Main Authors: Laouar, Leila, Fishbein, Ken, McGann, Locksley E., Horton, Walter E., Spencer, Richard G., Jomha, Nadr M.
Format: Article
Language:English
Subjects:
Animals
Articular cartilage
Biochemical
Cartilage, Articular - anatomy & histology
Cartilage, Articular - metabolism
Chondroitin Sulfates - metabolism
Cryopreservation
Cryopreservation - methods
Dimethyl sulfoxide
Freezing
Hydroxyproline - metabolism
Image Processing, Computer-Assisted
Magnetic Resonance Imaging
MRI
Porcine
Swine
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Summary:The objective of this study was to investigate the effects of cryopreservation on the components of articular cartilage (AC) matrix by utilizing magnetic resonance imaging (MRI) and biochemical assessments. Porcine AC (10 mm osteochondral dowels) was collected into four groups – (1) phosphate buffered saline (PBS) control, (2) PBS snap frozen in liquid nitrogen, (3) slow-cooled in dimethyl sulfoxide (DMSO), and (4) slow cooled in PBS (in absence of DMSO). MRI results demonstrated three distinct zones in the cartilage. After exposure to ice formation during cryopreservation procedures, alterations in MRI determined matrix fixed charged density and magnetization transfer rate were noted. In addition, biochemical assays demonstrated significant alterations in chondroitin sulfate and hydroxyproline content over time without differences in hydration or DNA content. In conclusion, MRI was able to detect some changes in the intact cartilage matrix structure consistent with biochemical assessments after ice formation during cryopreservation of intact porcine AC. Furthermore, biochemical assessments supported some of these findings and changed significantly after incubating the cartilage matrix for 36–72 h in PBS in terms of chondroitin sulfate and hydroxyproline content.
ISSN:0011-2240
1090-2392
DOI:10.1016/j.cryobiol.2006.10.193