Quality evaluation of Polygala japonica through simultaneous determination of six bioactive triterpenoid saponins by HPLC-ELSD

Polygala japonica Houtt (Polygalaceae), a traditional Chinese herb, has been used as expectorant, anti-inflammatory, antibacterial and antidepressant agent. To evaluate the quality of P. japonica, a high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) metho...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2007-03, Vol.43 (4), p.1552-1556
Main Authors: Wang, Honglan, Gao, Jie, Zhu, Danni, Yu, Boyang
Format: Article
Language:English
Subjects:
Analysis
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Drugs, Chinese Herbal - chemistry
Fundamental and applied biological sciences. Psychology
General pharmacology
HPLC-ELSD
Light
Medical sciences
Molecular Structure
Pharmacology. Drug treatments
Polygala - chemistry
Polygala japonica (Polygalaceae)
Quality Control
Quantification
Reproducibility of Results
Saponins - analysis
Saponins - chemistry
Scattering, Radiation
Sensitivity and Specificity
Temperature
Triterpenoid saponins
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Summary:Polygala japonica Houtt (Polygalaceae), a traditional Chinese herb, has been used as expectorant, anti-inflammatory, antibacterial and antidepressant agent. To evaluate the quality of P. japonica, a high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) method was developed for the simultaneous determination of six active triterpenoid saponins. With a Discovery C 18 analytical column, the analytes were separated efficiently using acetonitrile–methanol–0.05% trifluoroacetic acid (TFA) as mobile phase in a gradient program. The evaporator tube temperature of ELSD was set at 105 °C, and with the nebulizing gas flow-rate of 2.6 l/min. All calibration curves showed good linear regression ( γ > 0.9991) within the tested range. Additionally, reproducibility for the quantification of six saponins in P. japonica with intra- and inter-day variations of less than 5.0% was observed. Quantification of the six active saponins in P. japonica from different locations was performed by this newly developed method, which provides a new tool for the assessment of quality of P. japonica.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2006.11.012