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Quality evaluation of Polygala japonica through simultaneous determination of six bioactive triterpenoid saponins by HPLC-ELSD
Polygala japonica Houtt (Polygalaceae), a traditional Chinese herb, has been used as expectorant, anti-inflammatory, antibacterial and antidepressant agent. To evaluate the quality of P. japonica, a high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) metho...
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| Published in: | Journal of pharmaceutical and biomedical analysis 2007-03, Vol.43 (4), p.1552-1556 |
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| cites | cdi_FETCH-LOGICAL-c384t-87bb98c7f6e3a2df9bfc0208260dcd5e9caa189264ecda60b85d3074d9b7da3e3 |
| container_end_page | 1556 |
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| container_title | Journal of pharmaceutical and biomedical analysis |
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| creator | Wang, Honglan Gao, Jie Zhu, Danni Yu, Boyang |
| description | Polygala japonica Houtt (Polygalaceae), a traditional Chinese herb, has been used as expectorant, anti-inflammatory, antibacterial and antidepressant agent. To evaluate the quality of
P. japonica, a high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) method was developed for the simultaneous determination of six active triterpenoid saponins. With a Discovery C
18 analytical column, the analytes were separated efficiently using acetonitrile–methanol–0.05% trifluoroacetic acid (TFA) as mobile phase in a gradient program. The evaporator tube temperature of ELSD was set at 105
°C, and with the nebulizing gas flow-rate of 2.6
l/min. All calibration curves showed good linear regression (
γ
>
0.9991) within the tested range. Additionally, reproducibility for the quantification of six saponins in
P. japonica with intra- and inter-day variations of less than 5.0% was observed. Quantification of the six active saponins in
P. japonica from different locations was performed by this newly developed method, which provides a new tool for the assessment of quality of
P. japonica. |
| doi_str_mv | 10.1016/j.jpba.2006.11.012 |
| format | article |
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P. japonica, a high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) method was developed for the simultaneous determination of six active triterpenoid saponins. With a Discovery C
18 analytical column, the analytes were separated efficiently using acetonitrile–methanol–0.05% trifluoroacetic acid (TFA) as mobile phase in a gradient program. The evaporator tube temperature of ELSD was set at 105
°C, and with the nebulizing gas flow-rate of 2.6
l/min. All calibration curves showed good linear regression (
γ
>
0.9991) within the tested range. Additionally, reproducibility for the quantification of six saponins in
P. japonica with intra- and inter-day variations of less than 5.0% was observed. Quantification of the six active saponins in
P. japonica from different locations was performed by this newly developed method, which provides a new tool for the assessment of quality of
P. japonica.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/j.jpba.2006.11.012</identifier><identifier>PMID: 17158014</identifier><identifier>CODEN: JPBADA</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Chromatography, High Pressure Liquid - methods ; Drugs, Chinese Herbal - chemistry ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; HPLC-ELSD ; Light ; Medical sciences ; Molecular Structure ; Pharmacology. Drug treatments ; Polygala - chemistry ; Polygala japonica (Polygalaceae) ; Quality Control ; Quantification ; Reproducibility of Results ; Saponins - analysis ; Saponins - chemistry ; Scattering, Radiation ; Sensitivity and Specificity ; Temperature ; Triterpenoid saponins</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2007-03, Vol.43 (4), p.1552-1556</ispartof><rights>2006 Elsevier B.V.</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-87bb98c7f6e3a2df9bfc0208260dcd5e9caa189264ecda60b85d3074d9b7da3e3</citedby><cites>FETCH-LOGICAL-c384t-87bb98c7f6e3a2df9bfc0208260dcd5e9caa189264ecda60b85d3074d9b7da3e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18583272$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17158014$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Honglan</creatorcontrib><creatorcontrib>Gao, Jie</creatorcontrib><creatorcontrib>Zhu, Danni</creatorcontrib><creatorcontrib>Yu, Boyang</creatorcontrib><title>Quality evaluation of Polygala japonica through simultaneous determination of six bioactive triterpenoid saponins by HPLC-ELSD</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>Polygala japonica Houtt (Polygalaceae), a traditional Chinese herb, has been used as expectorant, anti-inflammatory, antibacterial and antidepressant agent. To evaluate the quality of
P. japonica, a high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) method was developed for the simultaneous determination of six active triterpenoid saponins. With a Discovery C
18 analytical column, the analytes were separated efficiently using acetonitrile–methanol–0.05% trifluoroacetic acid (TFA) as mobile phase in a gradient program. The evaporator tube temperature of ELSD was set at 105
°C, and with the nebulizing gas flow-rate of 2.6
l/min. All calibration curves showed good linear regression (
γ
>
0.9991) within the tested range. Additionally, reproducibility for the quantification of six saponins in
P. japonica with intra- and inter-day variations of less than 5.0% was observed. Quantification of the six active saponins in
P. japonica from different locations was performed by this newly developed method, which provides a new tool for the assessment of quality of
P. japonica.</description><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Drugs, Chinese Herbal - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>HPLC-ELSD</subject><subject>Light</subject><subject>Medical sciences</subject><subject>Molecular Structure</subject><subject>Pharmacology. Drug treatments</subject><subject>Polygala - chemistry</subject><subject>Polygala japonica (Polygalaceae)</subject><subject>Quality Control</subject><subject>Quantification</subject><subject>Reproducibility of Results</subject><subject>Saponins - analysis</subject><subject>Saponins - chemistry</subject><subject>Scattering, Radiation</subject><subject>Sensitivity and Specificity</subject><subject>Temperature</subject><subject>Triterpenoid saponins</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNp9kE1v1DAQhi0EokvhD3BAvsAtwXY-7Ehc0LZQpJUoAiRu1sSetI6SONjOir3w28myK3rjNId53lczDyEvOcs54_XbPu_nFnLBWJ1znjMuHpENV7LIRF3-eEw2TBY8k0xVF-RZjD1jrOJN-ZRccMkrxXi5Ib-_LDC4dKC4h2GB5PxEfUdv_XC4gwFoD7OfnAGa7oNf7u5pdOMyJJjQL5FaTBhGN_3LRfeLts6DSW6PNAW37mecvLM0_m2aIm0P9OZ2t82ud1-vnpMnHQwRX5znJfn-4frb9ibbff74aft-l5lClSlTsm0bZWRXYwHCdk3bGSaYEjWzxlbYGACumvVtNBZq1qrKFkyWtmmlhQKLS_Lm1DsH_3PBmPToosFhOD2iJRO8YkW1guIEmuBjDNjpObgRwkFzpo_Wda-P1vXRuuZcr9bX0Ktz-9KOaB8iZ80r8PoMQDQwdAEm4-IDpypVCHksenficHWxdxh0NA4ng9YFNElb7_53xx92OKOL</recordid><startdate>20070312</startdate><enddate>20070312</enddate><creator>Wang, Honglan</creator><creator>Gao, Jie</creator><creator>Zhu, Danni</creator><creator>Yu, Boyang</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20070312</creationdate><title>Quality evaluation of Polygala japonica through simultaneous determination of six bioactive triterpenoid saponins by HPLC-ELSD</title><author>Wang, Honglan ; Gao, Jie ; Zhu, Danni ; Yu, Boyang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-87bb98c7f6e3a2df9bfc0208260dcd5e9caa189264ecda60b85d3074d9b7da3e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Drugs, Chinese Herbal - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>HPLC-ELSD</topic><topic>Light</topic><topic>Medical sciences</topic><topic>Molecular Structure</topic><topic>Pharmacology. Drug treatments</topic><topic>Polygala - chemistry</topic><topic>Polygala japonica (Polygalaceae)</topic><topic>Quality Control</topic><topic>Quantification</topic><topic>Reproducibility of Results</topic><topic>Saponins - analysis</topic><topic>Saponins - chemistry</topic><topic>Scattering, Radiation</topic><topic>Sensitivity and Specificity</topic><topic>Temperature</topic><topic>Triterpenoid saponins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Honglan</creatorcontrib><creatorcontrib>Gao, Jie</creatorcontrib><creatorcontrib>Zhu, Danni</creatorcontrib><creatorcontrib>Yu, Boyang</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Honglan</au><au>Gao, Jie</au><au>Zhu, Danni</au><au>Yu, Boyang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quality evaluation of Polygala japonica through simultaneous determination of six bioactive triterpenoid saponins by HPLC-ELSD</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2007-03-12</date><risdate>2007</risdate><volume>43</volume><issue>4</issue><spage>1552</spage><epage>1556</epage><pages>1552-1556</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><coden>JPBADA</coden><abstract>Polygala japonica Houtt (Polygalaceae), a traditional Chinese herb, has been used as expectorant, anti-inflammatory, antibacterial and antidepressant agent. To evaluate the quality of
P. japonica, a high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) method was developed for the simultaneous determination of six active triterpenoid saponins. With a Discovery C
18 analytical column, the analytes were separated efficiently using acetonitrile–methanol–0.05% trifluoroacetic acid (TFA) as mobile phase in a gradient program. The evaporator tube temperature of ELSD was set at 105
°C, and with the nebulizing gas flow-rate of 2.6
l/min. All calibration curves showed good linear regression (
γ
>
0.9991) within the tested range. Additionally, reproducibility for the quantification of six saponins in
P. japonica with intra- and inter-day variations of less than 5.0% was observed. Quantification of the six active saponins in
P. japonica from different locations was performed by this newly developed method, which provides a new tool for the assessment of quality of
P. japonica.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>17158014</pmid><doi>10.1016/j.jpba.2006.11.012</doi><tpages>5</tpages></addata></record> |
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| language | eng |
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| subjects | Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Chromatography, High Pressure Liquid - methods Drugs, Chinese Herbal - chemistry Fundamental and applied biological sciences. Psychology General pharmacology HPLC-ELSD Light Medical sciences Molecular Structure Pharmacology. Drug treatments Polygala - chemistry Polygala japonica (Polygalaceae) Quality Control Quantification Reproducibility of Results Saponins - analysis Saponins - chemistry Scattering, Radiation Sensitivity and Specificity Temperature Triterpenoid saponins |
| title | Quality evaluation of Polygala japonica through simultaneous determination of six bioactive triterpenoid saponins by HPLC-ELSD |
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