Dopaminergic control of foregut contractions in Locusta migratoria

• Published in: Journal of insect physiology 2008, Vol.54 (1), p.222-230
• Main Authors: Lange, Angela B., Chan, Kenny
• Format: Article
• Language: English
• Subjects: amine and amino acid oxidoreductases; Animals; Chromatography, High Pressure Liquid; Dopamine; Dopamine - metabolism; Dopamine - pharmacology; dose response; Foregut; ganglia; Ganglia, Invertebrate - physiology; HPLC; Immunohistochemistry; innervation; Locusta migratoria; Locusta migratoria - physiology; locusts; muscle contraction; Muscle Contraction - drug effects; Muscle Contraction - physiology; Optic Lobe, Nonmammalian - physiology; physiological regulation; tyrosine hydroxylase; Upper Gastrointestinal Tract - innervation; Upper Gastrointestinal Tract - physiology; ventricular ganglia; Ventricular ganglion; Visceral muscle
• ISSN: 0022-1910; 1879-1611
• DOI: 10.1016/j.jinsphys.2007.09.005

Tyrosine hydroxylase-like immunoreactivity is present in cell bodies and processes in the brain and optic lobes of Locusta migratoria, with processes projecting along the frontal connectives to form a neuropile within the frontal ganglion. Immunoreactive cell bodies and processes are also evident in the hypocerebral and ventricular ganglia with processes extending over the foregut. Tyrosine hydroxylase is the rate-limiting enzyme in dopamine biosynthesis, and high-performance liquid chromatography coupled to electrochemical detection was used to confirm the presence of dopamine in the innervation to the foregut. Spontaneous foregut contractions are under the control of the ventricular ganglia and are absent when these ganglia are removed. Dopamine leads to an inhibition of both the amplitude and frequency of phasic contractions of the foregut that are produced when the ventricular ganglia are left attached. Dopamine has direct effects on the foregut muscle in the absence of the ventricular ganglia, inhibiting a proctolin-induced contraction in a dose-dependent manner.