Role of Phospholipase A2 in the Induction of Drip Loss in Porcine Muscle

The role of phospholipase A2 in the induction of drip loss from pig muscle has been investigated. In samples from porcine M. longissimus dorsi, total PLA2 activity as well as mRNA and protein levels of the group VIA iPLA2 (iPLA2-VIA) increased during the initial 4 h post-mortem period. Morphological...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2007-03, Vol.55 (5), p.1970-1976
Main Authors: Poulsen, Kristian A, Young, Jette F, Theil, Peter, Kolko, Miriam, Oksbjerg, Niels, Lambert, Ian H
Format: Article
Language:English
Subjects:
Animal productions
Animals
Biological and medical sciences
Body Water - physiology
cell membranes
drip loss
enzyme activity
Food industries
Fundamental and applied biological sciences. Psychology
gene expression
Hydrogen-Ion Concentration
meat quality
membrane permeability
messenger RNA
muscle fiber membranes
muscle fibers
Muscle, Skeletal - enzymology
Muscle, Skeletal - physiology
phospholipase A2
Phospholipases A - analysis
Phospholipases A - genetics
Phospholipases A - metabolism
Phospholipases A2
pork
Postmortem Changes
RNA, Messenger - analysis
Swine
Terrestrial animal productions
Vertebrates
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Summary:The role of phospholipase A2 in the induction of drip loss from pig muscle has been investigated. In samples from porcine M. longissimus dorsi, total PLA2 activity as well as mRNA and protein levels of the group VIA iPLA2 (iPLA2-VIA) increased during the initial 4 h post-mortem period. Morphological studies of porcine muscle showed that at 4 h post-mortem, gaps had formed between muscle fibers and that the sarcolemma membrane borders appeared blurred. At the same time iPLA2-VIA protein levels were increased inside muscle fibers and at the sarcolemma. iPLA2-VIA mRNA abundance in samples from different breeds of pigs with variations in drip loss revealed no clear correlation between drip loss level and iPLA2-VIA expression. Together, these data indicate that during the post-mortem period, iPLA2-VIA expression and activity is increased at the muscle fiber membranes. PLA2 activity may affect membrane permeability and consequently the progression of drip formation in porcine muscle. Keywords: Water-holding capacity; phospholipase A2; pig muscle; bromoenol lactone; iPLA2-VIA; C2C12 myotubes; iPLA2-VIB
ISSN:0021-8561
1520-5118
DOI:10.1021/jf062341n