High prevalence of Cryptosporidium bovis and the deer-like genotype in calves compared to mature cows in beef cow-calf operations

• Published in: Veterinary parasitology 2008-02, Vol.151 (2), p.191-195
• Main Authors: Feltus, Dawn C., Giddings, Catherine W., Khaitsa, Margaret L., McEvoy, John M.
• Format: Article
• Language: English
• Subjects: animal age; animal pathogens; Animals; Base Sequence; Beef; beef cattle; beef cows; Calves; Cattle; Cattle Diseases - epidemiology; Cattle Diseases - parasitology; cow-calf operations; Cows; cryptosporidiosis; Cryptosporidiosis - classification; Cryptosporidiosis - epidemiology; Cryptosporidiosis - veterinary; Cryptosporidium; Cryptosporidium - genetics; Cryptosporidium - isolation & purification; Cryptosporidium andersoni; Cryptosporidium bovis; Cryptosporidium deer-like genotype; disease prevalence; Female; Genotype; Molecular Sequence Data; North Dakota - epidemiology; nucleotide sequences; Polymorphism, Restriction Fragment Length; Prevalence; restriction fragment length polymorphism; ribosomal RNA; RNA, Ribosomal, 18S - genetics; sequence analysis; strain differences
• ISSN: 0304-4017; 1873-2550
• DOI: 10.1016/j.vetpar.2007.10.012

Recent studies have identified the novel, host adapted Cryptosporidium bovis and the deer-like genotype in dairy cattle from farms in the United States, China, India and Europe. This novel species and genotype appear to be more prevalent in older, post-weaned dairy cattle than previously thought. However, little information is available on their prevalence in beef cow-calf operations. In the present study, we determined the prevalence of Cryptosporidium species in 98 calves (6–8 months old) and 114 cows (>2 years old) in seven beef cow-calf herds in western North Dakota. DNA was extracted from fecal samples and Cryptosporidium spp. were identified by amplification of the 18S rRNA gene followed by sequencing or RFLP analysis. All seven herds tested positive for Cryptosporidium. Overall, 43/212 (20.3%) animals were positive. Only five of these positives were from cows. C. bovis, the deer-like genotype and C. andersoni were identified in 9.4, 6.6 and 1.4% of animals sampled, respectively. C. parvum was not identified in any of the positive samples. C. bovis, the deer-like genotype and C. andersoni were detected in 6/7, 5/7 and 2/7 herds, respectively. C. bovis and the deer-like genotype were primarily detected in calves, while C. andersoni was only detected in cows. Six isolates could not be typed. These results show a relatively high prevalence of C. bovis and the deer-like genotype in 6–8-month-old beef calves compared to cows older than 2 years in the seven herds studied.