Liquefaction of lignocellulose at high-solids concentrations

• Published in: Biotechnology and bioengineering 2007-04, Vol.96 (5), p.862-870
• Main Authors: Jørgensen, Henning, Vibe-Pedersen, Jakob, Larsen, Jan, Felby, Claus
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Biomass; Bioreactors - microbiology; Biotechnology; Carbohydrate Metabolism; Cellulase - metabolism; Cellulose - metabolism; enzymatic hydrolysis; Enzymes; Ethanol; Ethanol - metabolism; Fermentation; Fundamental and applied biological sciences. Psychology; Lignin - metabolism; Saccharomyces cerevisiae; Saccharomyces cerevisiae - metabolism; Trichoderma - metabolism; Triticum - metabolism; Triticum aestivum; wheat straw; Yeast
• ISSN: 0006-3592; 1097-0290
• DOI: 10.1002/bit.21115

To improve process economics of the lignocellulose to ethanol process a reactor system for enzymatic liquefaction and saccharification at high‐solids concentrations was developed. The technology is based on free fall mixing employing a horizontally placed drum with a horizontal rotating shaft mounted with paddlers for mixing. Enzymatic liquefaction and saccharification of pretreated wheat straw was tested with up to 40% (w/w) initial DM. In less than 10 h, the structure of the material was changed from intact straw particles (length 1–5 cm) into a paste/liquid that could be pumped. Tests revealed no significant effect of mixing speed in the range 3.3–11.5 rpm on the glucose conversion after 24 h and ethanol yield after subsequent fermentation for 48 h. Low‐power inputs for mixing are therefore possible. Liquefaction and saccharification for 96 h using an enzyme loading of 7 FPU/g·DM and 40% DM resulted in a glucose concentration of 86 g/kg. Experiments conducted at 2%–40% (w/w) initial DM revealed that cellulose and hemicellulose conversion decreased almost linearly with increasing DM. Performing the experiments as simultaneous saccharification and fermentation also revealed a decrease in ethanol yield at increasing initial DM. Saccharomyces cerevisiae was capable of fermenting hydrolysates up to 40% DM. The highest ethanol concentration, 48 g/kg, was obtained using 35% (w/w) DM. Liquefaction of biomass with this reactor system unlocks the possibility of 10% (w/w) ethanol in the fermentation broth in future lignocellulose to ethanol plants. Biotechnol. Bioeng. 2007;96:862–870. © 2006 Wiley Periodicals, Inc.