Involvement of accumulated NOS inhibitors and endothelin-1, enhanced arginase, and impaired DDAH activities in pulmonary dysfunction following subarachnoid hemorrhage in the rabbit

• Published in: Vascular pharmacology 2008-01, Vol.48 (1), p.21-31
• Main Authors: Mizuno, Yusuke, Isotani, Eiji, Ohno, Kikuo, Nagai, Akiko, Imamura, Masatoshi, Azuma, Hiroshi
• Format: Article
• Language: English
• Subjects: Acetylcholine - pharmacology; Amidohydrolases - metabolism; Animals; Arginase; Arginase - metabolism; Cardiovascular; Cyclic GMP - metabolism; Cyclooxygenase Inhibitors - pharmacology; DDAH; Dose-Response Relationship, Drug; Endogenous NOS inhibitors; Endothelin-1 - metabolism; Experimental SAH; Indomethacin - pharmacology; Lung Diseases - etiology; Lung Diseases - metabolism; Male; Models, Biological; Nitric Oxide Donors - pharmacology; Nitric Oxide Synthase - antagonists & inhibitors; Nitric Oxide Synthase - metabolism; Nitroarginine - pharmacology; Nitroprusside - pharmacology; omega-N-Methylarginine - metabolism; omega-N-Methylarginine - pharmacology; Oxadiazoles - antagonists & inhibitors; Oxadiazoles - pharmacology; Pulmonary Artery - drug effects; Pulmonary Artery - metabolism; Pulmonary Artery - physiopathology; Pulmonary dysfunction; Quinoxalines - antagonists & inhibitors; Quinoxalines - pharmacology; Rabbits; Rats; Rats, Sprague-Dawley; Subarachnoid Hemorrhage - complications; Vasoconstriction - drug effects
• ISSN: 1537-1891; 1879-3649
• DOI: 10.1016/j.vph.2007.11.002

Abstract We designed the present experiments to investigate the involvement of endogenous nitric oxide synthase (NOS) inhibitors, dimethylarginine dimethylaminohydrolase (DDAH) as a hydrolyzing enzyme of the NOS inhibitors, NOS, arginase which shares l -arginine as a common substrate with NOS, and endothelin-1 (ET-1) in the pulmonary dysfunction after induction of experimental subarachnoid hemorrhage (SAH) in the rabbit. SAH was induced by injecting autologous blood into the cisterna magna, and controls were injected with saline. On day 2, pulmonary arteries were isolated for determinations. A significant impairment of the endothelium-dependent relaxation (EDR) caused by acetylcholine was found in 20 cases (43.5%) out of 46 SAH animals, and the same animals exhibited accompanying the significantly impaired cyclic GMP production, accumulated endogenous NOS inhibitors, attenuated DDAH activity, enhanced arginase activity and accumulated ET-1 within the vessel wall. Meanwhile, there were no differences in endothelial NOS activity per se and sodium nitroprusside-induced relaxation between the animals with an impaired EDR and those without such a change. ET-1 content within aortic wall was increased with concomitant decrease in cyclic GMP production after the intraperitoneal application of authentic monomethylarginine as a NOS inhibitor in the rat. The current results suggest that accumulated endogenous NOS inhibitors and enhanced arginase activity possibly bring about the impaired NO production, thereby attenuating the EDR and contributing to the accumulation of ET-1 within the vessel wall. The accumulated endogenous NOS inhibitors at least partly result from the decreased DDAH activity. These alterations may be relevant to the pulmonary dysfunction after induction of SAH.