Virus-induced gene silencing for functional analysis of selected genes

Virus-induced gene silencing (VIGS) is a technology that exploits an RNA-mediated antiviral defense mechanism and has been shown to be of great potential in plant reverse genetics. Circumvention of plant transformation, methodological simplicity, robustness, and speedy results makes VIGS an attracti...

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Bibliographic Details
Published in:Plant cell reports 2008-02, Vol.27 (2), p.209-219
Main Authors: GODGE, Mandar R, PURKAYASTHA, Arunima, DASGUPTA, Indranil, KUMAR, Prakash P
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Diverse techniques
Fundamental and applied biological sciences. Psychology
Gene Silencing
Genetic Vectors - genetics
Genomics - methods
Models, Biological
Molecular and cellular biology
Nicotiana - genetics
Nicotiana - virology
Nicotiana benthamiana
Plant Viruses - physiology
Plants - genetics
Plants - virology
Plants, Genetically Modified
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Summary:Virus-induced gene silencing (VIGS) is a technology that exploits an RNA-mediated antiviral defense mechanism and has been shown to be of great potential in plant reverse genetics. Circumvention of plant transformation, methodological simplicity, robustness, and speedy results makes VIGS an attractive alternative instrument in functional genomics, even in a high throughput fashion. The system is well established in Nicotiana benthamiana, and efforts are being made to improve VIGS in other species, including monocots. Here, we discuss the issues specific to the application of VIGS technology to determine gene function, which has revealed the roles of a variety of genes in disease resistance, abiotic stress, cellular signaling and secondary metabolite biosynthesis.
ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-007-0460-2