Using Capacitance Measurements as the Detection Method in Antigen-Containing Layer-by-Layer Films for Biosensing

The layer-by-layer technique is employed here to immobilize antigen-containing liposomes, so-called proteoliposomes, onto Au-interdigitated substrates, which are capable of molecular recognition of anti-pasteurellosis antibodies. Detection was carried out using a novel strategy entirely based upon c...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2007-03, Vol.79 (5), p.2163-2167
Main Authors: Zucolotto, Valtencir, Daghastanli, Katia R. P, Hayasaka, Caio O, Riul, Antonio, Ciancaglini, Pietro, Oliveira, Osvaldo N
Format: Article
Language:English
Subjects:
Analytical chemistry
Antibodies, Bacterial - blood
Antigens
Antigens - chemistry
Antigens - immunology
Biological and medical sciences
Biosensing Techniques - methods
Biosensors
Biotechnology
Cysts
Electric Capacitance
Electrodes
Fundamental and applied biological sciences. Psychology
Gold - chemistry
Humans
Immunoglobulin G - blood
Immunoglobulins
Membranes
Methods. Procedures. Technologies
Molecules
Pasteurella Infections - diagnosis
Protein Array Analysis - methods
Proteolipids - chemistry
Various methods and equipments
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Summary:The layer-by-layer technique is employed here to immobilize antigen-containing liposomes, so-called proteoliposomes, onto Au-interdigitated substrates, which are capable of molecular recognition of anti-pasteurellosis antibodies. Detection was carried out using a novel strategy entirely based upon capacitance measurements, and to enhance sensitivity, we combine the response of three different sensing units in a similar procedure used for taste sensors. With the three-electrode array immunoglobulin G (IgG) against pasteurellosis is detected at concentrations as low as nanograms per milliliter. Furthermore, because of the molecular recognition capability, a distinction can be made between specific and nonspecific IgG. The concepts behind the biosensors reported here may have a large impact for clinical tests, as the procedures to detect the antibody take only a few minutes and the biosensors are relatively low cost.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac0616153