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Using Capacitance Measurements as the Detection Method in Antigen-Containing Layer-by-Layer Films for Biosensing
The layer-by-layer technique is employed here to immobilize antigen-containing liposomes, so-called proteoliposomes, onto Au-interdigitated substrates, which are capable of molecular recognition of anti-pasteurellosis antibodies. Detection was carried out using a novel strategy entirely based upon c...
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| Published in: | Analytical chemistry (Washington) 2007-03, Vol.79 (5), p.2163-2167 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-a439t-e5d875c0d46b19434ad4a4e4f4d7661d173141c06ca234dcd593ee15668656a13 |
| container_end_page | 2167 |
| container_issue | 5 |
| container_start_page | 2163 |
| container_title | Analytical chemistry (Washington) |
| container_volume | 79 |
| creator | Zucolotto, Valtencir Daghastanli, Katia R. P Hayasaka, Caio O Riul, Antonio Ciancaglini, Pietro Oliveira, Osvaldo N |
| description | The layer-by-layer technique is employed here to immobilize antigen-containing liposomes, so-called proteoliposomes, onto Au-interdigitated substrates, which are capable of molecular recognition of anti-pasteurellosis antibodies. Detection was carried out using a novel strategy entirely based upon capacitance measurements, and to enhance sensitivity, we combine the response of three different sensing units in a similar procedure used for taste sensors. With the three-electrode array immunoglobulin G (IgG) against pasteurellosis is detected at concentrations as low as nanograms per milliliter. Furthermore, because of the molecular recognition capability, a distinction can be made between specific and nonspecific IgG. The concepts behind the biosensors reported here may have a large impact for clinical tests, as the procedures to detect the antibody take only a few minutes and the biosensors are relatively low cost. |
| doi_str_mv | 10.1021/ac0616153 |
| format | article |
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P ; Hayasaka, Caio O ; Riul, Antonio ; Ciancaglini, Pietro ; Oliveira, Osvaldo N</creator><creatorcontrib>Zucolotto, Valtencir ; Daghastanli, Katia R. P ; Hayasaka, Caio O ; Riul, Antonio ; Ciancaglini, Pietro ; Oliveira, Osvaldo N</creatorcontrib><description>The layer-by-layer technique is employed here to immobilize antigen-containing liposomes, so-called proteoliposomes, onto Au-interdigitated substrates, which are capable of molecular recognition of anti-pasteurellosis antibodies. Detection was carried out using a novel strategy entirely based upon capacitance measurements, and to enhance sensitivity, we combine the response of three different sensing units in a similar procedure used for taste sensors. With the three-electrode array immunoglobulin G (IgG) against pasteurellosis is detected at concentrations as low as nanograms per milliliter. Furthermore, because of the molecular recognition capability, a distinction can be made between specific and nonspecific IgG. The concepts behind the biosensors reported here may have a large impact for clinical tests, as the procedures to detect the antibody take only a few minutes and the biosensors are relatively low cost.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac0616153</identifier><identifier>PMID: 17263514</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Analytical chemistry ; Antibodies, Bacterial - blood ; Antigens ; Antigens - chemistry ; Antigens - immunology ; Biological and medical sciences ; Biosensing Techniques - methods ; Biosensors ; Biotechnology ; Cysts ; Electric Capacitance ; Electrodes ; Fundamental and applied biological sciences. Psychology ; Gold - chemistry ; Humans ; Immunoglobulin G - blood ; Immunoglobulins ; Membranes ; Methods. Procedures. Technologies ; Molecules ; Pasteurella Infections - diagnosis ; Protein Array Analysis - methods ; Proteolipids - chemistry ; Various methods and equipments</subject><ispartof>Analytical chemistry (Washington), 2007-03, Vol.79 (5), p.2163-2167</ispartof><rights>Copyright © 2007 American Chemical Society</rights><rights>2008 INIST-CNRS</rights><rights>Copyright American Chemical Society Mar 1, 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a439t-e5d875c0d46b19434ad4a4e4f4d7661d173141c06ca234dcd593ee15668656a13</citedby><cites>FETCH-LOGICAL-a439t-e5d875c0d46b19434ad4a4e4f4d7661d173141c06ca234dcd593ee15668656a13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19085249$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17263514$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zucolotto, Valtencir</creatorcontrib><creatorcontrib>Daghastanli, Katia R. P</creatorcontrib><creatorcontrib>Hayasaka, Caio O</creatorcontrib><creatorcontrib>Riul, Antonio</creatorcontrib><creatorcontrib>Ciancaglini, Pietro</creatorcontrib><creatorcontrib>Oliveira, Osvaldo N</creatorcontrib><title>Using Capacitance Measurements as the Detection Method in Antigen-Containing Layer-by-Layer Films for Biosensing</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>The layer-by-layer technique is employed here to immobilize antigen-containing liposomes, so-called proteoliposomes, onto Au-interdigitated substrates, which are capable of molecular recognition of anti-pasteurellosis antibodies. Detection was carried out using a novel strategy entirely based upon capacitance measurements, and to enhance sensitivity, we combine the response of three different sensing units in a similar procedure used for taste sensors. With the three-electrode array immunoglobulin G (IgG) against pasteurellosis is detected at concentrations as low as nanograms per milliliter. Furthermore, because of the molecular recognition capability, a distinction can be made between specific and nonspecific IgG. The concepts behind the biosensors reported here may have a large impact for clinical tests, as the procedures to detect the antibody take only a few minutes and the biosensors are relatively low cost.</description><subject>Analytical chemistry</subject><subject>Antibodies, Bacterial - blood</subject><subject>Antigens</subject><subject>Antigens - chemistry</subject><subject>Antigens - immunology</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Cysts</subject><subject>Electric Capacitance</subject><subject>Electrodes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gold - chemistry</subject><subject>Humans</subject><subject>Immunoglobulin G - blood</subject><subject>Immunoglobulins</subject><subject>Membranes</subject><subject>Methods. Procedures. 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| ispartof | Analytical chemistry (Washington), 2007-03, Vol.79 (5), p.2163-2167 |
| issn | 0003-2700 1520-6882 |
| language | eng |
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| source | American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list) |
| subjects | Analytical chemistry Antibodies, Bacterial - blood Antigens Antigens - chemistry Antigens - immunology Biological and medical sciences Biosensing Techniques - methods Biosensors Biotechnology Cysts Electric Capacitance Electrodes Fundamental and applied biological sciences. Psychology Gold - chemistry Humans Immunoglobulin G - blood Immunoglobulins Membranes Methods. Procedures. Technologies Molecules Pasteurella Infections - diagnosis Protein Array Analysis - methods Proteolipids - chemistry Various methods and equipments |
| title | Using Capacitance Measurements as the Detection Method in Antigen-Containing Layer-by-Layer Films for Biosensing |
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