Is there a role for PCR-SSCP among the methods for missense mutation detection of TP53 gene?

Mutation analysis methods have increased in variety during the past years. High-throughput microarray methods have especially increased in popularity. However, new methods require reference points, and not all of the methods are equal in sensitivity and specificity. Furthermore, the detection of unk...

Full description

Saved in:
Bibliographic Details
Published in:Human & experimental toxicology 2007-01, Vol.26 (1), p.9-18
Main Authors: Peltonen, J, Welsh, J A, Vähäkangas, K H
Format: Article
Language:English
Subjects:
Biological and medical sciences
Breast cancer
Carcinogens
Chemical mutagenesis
Chemotherapy
Genes, p53
Humans
Lung cancer
Medical research
Medical sciences
Mutation
Mutation, Missense
Pharmacology
Plasma
Polymerase Chain Reaction - methods
Polymorphism
Polymorphism, Single-Stranded Conformational
Toxicology
Tumor Suppressor Protein p53 - genetics
Tumors
Citations: Items that this one cites
Items that cite this one
Online Access:Request full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Mutation analysis methods have increased in variety during the past years. High-throughput microarray methods have especially increased in popularity. However, new methods require reference points, and not all of the methods are equal in sensitivity and specificity. Furthermore, the detection of unknown missense mutations, such as unknown TP53 mutations in human tumors, for clinical purposes requires great accuracy, which may be difficult to acquire with the current high-throughput methods. For these reasons, the classical methods, such as PCR-manual sequencing and PCR-SSCP, are still valuable and necessary.
ISSN:0960-3271
1477-0903
DOI:10.1177/0960327107071918