Mumps virus reinfection is not a rare event confirmed by reverse transcription loop-mediated isothermal amplification

• Published in: Journal of medical virology 2008-03, Vol.80 (3), p.517-523
• Main Authors: Yoshida, Naoko, Fujino, Motoko, Miyata, Akiko, Nagai, Takao, Kamada, Makoto, Sakiyama, Hiroshi, Ihara, Toshiaki, Kumagai, Takuji, Okafuji, Teruo, Okafuji, Takao, Nakayama, Tetsuo
• Format: Article
• Language: English
• Subjects: Antibodies, Viral - blood; Antibodies, Viral - immunology; avidity tests; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Genome, Viral; Genotype; Human viral diseases; Humans; IgG EIA; IgM EIA; Infectious diseases; Medical sciences; Microbiology; Miscellaneous; Mumps - diagnosis; Mumps - immunology; Mumps - virology; Mumps Vaccine - immunology; Mumps virus; Mumps virus - genetics; Mumps virus - immunology; Mumps virus - isolation & purification; Mumps virus - physiology; Nucleic Acid Amplification Techniques - methods; Recurrence; reinfection; vaccine failure; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Viral diseases; Viral Load; Virology
• ISSN: 0146-6615; 1096-9071
• DOI: 10.1002/jmv.21106

Clinically apparent mumps reinfection is considered extremely rare, but several cases have been suspected of reinfection in an out-patient clinic. In this study, virological examination, virus isolation, the reverse transcription loop-mediated isothermal amplification (RT-LAMP), and IgG and IgM EIA antibodies, were examined in order to identify mumps reinfection. Patients were divided into three categories; the reinfection group comprised 29 patients with a history of natural infection, the vaccine-failure group consisted of 37 patients with an immunization history, and two patients had histories of both immunization and mumps infection. Another 25 patients were enrolled as a primary infection group. Mumps virus was isolated in 5 (17%) and the genome was detected in 12 (41%) of 29 in the reinfection group. Reinfection was confirmed in 21/28, demonstrating high avidity of IgG EIA. Mumps virus was isolated in 15 (41%) and there was a higher positivity of genome amplification in 25 (68%) of 37 patients in the vaccine-failure group. Among these, 23 were confirmed as secondary vaccine failure by high avidity IgG EIA serology. In the primary infection group, the isolation rate and genome detection rate was higher in 16 (64%) and in 18 (72%) of 25 patients, respectively. There was no significant difference in virus load among the three groups but high mumps virus load was suspected in the IgM EIA-positive group based on the shorter amplification time on RT-LAMP. Mumps virus reinfection was confirmed by RT-LAMP and an IgG avidity test and was not a rare event. J. Med. Virol. 80:517-523, 2008. © 2008 Wiley-Liss, Inc.