A Metabolomic Study of Brain Tissues from Aged Mice with Low Expression of the Vesicular Monoamine Transporter 2 (VMAT2) Gene

The vesicular monoamine transporter 2 (VMAT2) sequesters monoamines into synaptic vesicles in preparation for neurotransmission. Samples of cerebellum, cortex, hippocampus, substantia nigra and striatum from VMAT2-deficient mice were compared to age-matched control mice. Multivariate statistical ana...

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Bibliographic Details
Published in:Neurochemical research 2008-02, Vol.33 (2), p.292-300
Main Authors: Salek, Reza M., Colebrooke, Rebecca E., Macintosh, Robin, Lynch, Patrick J., Sweatman, Brian C., Emson, Piers C., Griffin, Julian L.
Format: Article
Language:English
Subjects:
Aging - metabolism
Animals
Biochemistry
Biomedical and Life Sciences
Biomedicine
Brain - metabolism
Cell Biology
Female
Male
Mice
Neurochemistry
Neurology
Neurosciences
Nuclear Magnetic Resonance, Biomolecular
Original Paper
Vesicular Monoamine Transport Proteins - genetics
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Summary:The vesicular monoamine transporter 2 (VMAT2) sequesters monoamines into synaptic vesicles in preparation for neurotransmission. Samples of cerebellum, cortex, hippocampus, substantia nigra and striatum from VMAT2-deficient mice were compared to age-matched control mice. Multivariate statistical analyses of 1 H NMR spectral profiles separated VMAT2-deficient mice from controls for all five brain regions. Although the data show that metabolic alterations are region- and age-specific, in general, analyses indicated decreases in the concentrations of taurine and creatine/phosphocreatine and increases in glutamate and N -acetyl aspartate in VMAT2-deficient mouse brain tissues. This study demonstrates the efficacy of metabolomics as a functional genomics phenotyping tool for mouse models of neurological disorders, and indicates that mild reductions in the expression of VMAT2 affect normal brain metabolism.
ISSN:0364-3190
1573-6903
DOI:10.1007/s11064-007-9542-3