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2‘,3‘-Dideoxy-3‘-thionucleoside Triphosphates: Syntheses and Polymerase Substrate Activities
All four 2‘,3‘-dideoxy-3‘-thio-nucleosides (ddtNTPs) function as substrates for the Y410F mutant of Deep Vent (exo-) DNA polymerase. Not only are the ddtNTPs incorporated to form the N + 1 product, but further elongations are observed in which the key step is attack of the 3‘-thiol on the 5‘-triphos...
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| Published in: | Organic letters 2007-03, Vol.9 (6), p.1161-1163 |
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| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-a313t-df4d273d7936710efa876772672e14e2107f55a62932599ab309021fae4c57903 |
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| cites | cdi_FETCH-LOGICAL-a313t-df4d273d7936710efa876772672e14e2107f55a62932599ab309021fae4c57903 |
| container_end_page | 1163 |
| container_issue | 6 |
| container_start_page | 1161 |
| container_title | Organic letters |
| container_volume | 9 |
| creator | Meena Sam, Mui Pierce, Kathryn Szostak, Jack W McLaughlin, Larry W |
| description | All four 2‘,3‘-dideoxy-3‘-thio-nucleosides (ddtNTPs) function as substrates for the Y410F mutant of Deep Vent (exo-) DNA polymerase. Not only are the ddtNTPs incorporated to form the N + 1 product, but further elongations are observed in which the key step is attack of the 3‘-thiol on the 5‘-triphosphate. Although other polymerases are likely to differ in their use of the ddtNTPs, there does not appear to be a fundamental prohibition against using a thiol nucleophile on a phosphate anhydride electrophile. The syntheses of four ddtNTPs (C, T, A, G) are described. |
| doi_str_mv | 10.1021/ol070147w |
| format | article |
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| fulltext | fulltext |
| identifier | ISSN: 1523-7060 |
| ispartof | Organic letters, 2007-03, Vol.9 (6), p.1161-1163 |
| issn | 1523-7060 1523-7052 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70236193 |
| source | American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list) |
| subjects | Catalysis DNA-Directed DNA Polymerase - metabolism Electrophoresis, Polyacrylamide Gel Enzyme Inhibitors - chemical synthesis Enzyme Inhibitors - pharmacology Mutation Nucleic Acid Synthesis Inhibitors Phosphates - chemistry Substrate Specificity Sulfhydryl Compounds - chemistry Thionucleosides - chemical synthesis |
| title | 2‘,3‘-Dideoxy-3‘-thionucleoside Triphosphates: Syntheses and Polymerase Substrate Activities |
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