Thymosinalpha1 stimulates cell proliferation by activating ERK1/2, JNK, and increasing cytokine secretion in human pancreatic cancer cells

In this study, we investigated the expression and function of thymosinalpha1 (Thyalpha1) in human pancreatic cancer. We found that human pancreatic cancer cell lines Panc-1, Panc03.27, ASPC-1, and PL45 cells significantly over-expressed the mRNA of Thyalpha1 as compared to the normal human pancreati...

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Bibliographic Details
Published in:Cancer letters 2007-04, Vol.248 (1), p.58-67
Main Authors: Li, Min, Feurino, Louis W, Li, Fei, Wang, Hao, Zhai, Qihui, Fisher, William E, Chen, Changyi, Yao, Qizhi
Format: Article
Language:English
Subjects:
Cell Line, Tumor
Cell Proliferation - drug effects
Cytokines - secretion
Dose-Response Relationship, Drug
Enzyme Activation - drug effects
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
Interleukin-10 - secretion
Interleukin-13 - secretion
Interleukin-17 - secretion
JNK Mitogen-Activated Protein Kinases - metabolism
Mitogen-Activated Protein Kinase 1 - metabolism
Mitogen-Activated Protein Kinase 3 - metabolism
Mitogen-Activated Protein Kinases - metabolism
Pancreatic Neoplasms - genetics
Pancreatic Neoplasms - metabolism
Pancreatic Neoplasms - pathology
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
Th2 Cells - metabolism
Thymosin - analogs & derivatives
Thymosin - genetics
Thymosin - metabolism
Thymosin - pharmacology
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Summary:In this study, we investigated the expression and function of thymosinalpha1 (Thyalpha1) in human pancreatic cancer. We found that human pancreatic cancer cell lines Panc-1, Panc03.27, ASPC-1, and PL45 cells significantly over-expressed the mRNA of Thyalpha1 as compared to the normal human pancreatic ductal epithelium (HPDE) cells.. Thyalpha1 mRNA and protein levels were also over-expressed in clinical pancreatic adenocarcinoma specimens. In addition, synthetic Thyalpha1 significantly promoted Panc-1 cell proliferation and increased phosphorylation of ERK1/2 and JNK. Furthermore, Thyalpha1 increased the secretion of multiple cytokines including IL-10, IL-13, and IL-17 in Panc-1 cells. Thus, Thyalpha1 may have a new role in pancreatic cancer pathogenesis.
ISSN:0304-3835