Probing RNA-antibiotic interactions: a FTIR study

The crystal structure determination of antibiotic binding sites on the 30S ribosomal subunit and the increasing demand for developing RNA-based drugs has prompted us to study the direct binding of spectinomycin, vancomycin and bleomycin with yeast total RNA using Fourier transform infrared (FTIR) sp...

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Bibliographic Details
Published in:Molecular biology reports 2008-03, Vol.35 (1), p.51-57
Main Authors: Prathiba, J, Malathi, R
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Anti-Bacterial Agents - metabolism
Anti-Bacterial Agents - pharmacology
Antibiotics
Binding sites
Biomedical and Life Sciences
Bleomycin
Crystal structure
FTIR
Histology
Life Sciences
Magnesium - pharmacology
Morphology
Original Paper
Peptides
Pharmacology
Ribonucleic acid
RNA
RNA, Fungal - metabolism
RNA-antibiotic interaction
Saccharomyces cerevisiae - drug effects
spectinomycin
Spectinomycin - metabolism
Spectinomycin - pharmacology
Spectroscopy, Fourier Transform Infrared
Vancomycin
Vancomycin - metabolism
Vancomycin - pharmacology
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Summary:The crystal structure determination of antibiotic binding sites on the 30S ribosomal subunit and the increasing demand for developing RNA-based drugs has prompted us to study the direct binding of spectinomycin, vancomycin and bleomycin with yeast total RNA using Fourier transform infrared (FTIR) spectroscopy. We report that the OH of spectinomycin and the peptide group of vancomycin can bind to the bases of RNA, which might depend on Mg²⁺ concentration. Bleomycin on the other hand does not show such a drastic effect on yeast total RNA. This study might help in developing innovative strategies utilizing RNA molecules to perform a variety of essential biological functions.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-006-9051-5