HUGO (FNDC3A): a New Gene Overexpressed in Human Odontoblasts

Previously, we established a subtractive cDNA library enriched in odontoblast-specific genes and hypothesized that new, previously unidentified, markers would be present, associated with the odontoblast phenotype. In this paper, we report the first characterization of a new gene we have named HUGO,...

Full description

Saved in:
Bibliographic Details
Published in:Journal of dental research 2008-02, Vol.87 (2), p.131-136
Main Authors: Carrouel, F., Couble, M.-L., Vanbelle, C., Staquet, M.-J., Magloire, H., Bleicher, F.
Format: Article
Language:English
Subjects:
Animals
Blotting, Northern
Blotting, Western
Cells, Cultured
Cercopithecus aethiops
Chromosomes, Human, Pair 13 - genetics
Conserved Sequence - genetics
COS Cells
Dental Pulp - cytology
Dental Pulp - innervation
Dentistry
Exons - genetics
Fibronectins - genetics
Flow Cytometry
Gene Expression Regulation - genetics
Golgi Apparatus - metabolism
Humans
Immunohistochemistry
Introns - genetics
Neoplasm Proteins - genetics
Odontoblasts - metabolism
Open Reading Frames - genetics
Polymerase Chain Reaction
Promoter Regions, Genetic - genetics
Protein Isoforms - genetics
Sequence Analysis, DNA
Structural Homology, Protein
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Previously, we established a subtractive cDNA library enriched in odontoblast-specific genes and hypothesized that new, previously unidentified, markers would be present, associated with the odontoblast phenotype. In this paper, we report the first characterization of a new gene we have named HUGO, and its associated deduced protein sequence. This gene expression is under the control of two alternative promoters, resulting in the synthesis of two proteins, one of which, HUGO2, is included in the other, HUGO1. HUGO proteins are mainly composed of a proline-rich region at the N-terminus, 8 type III-fibronectin modules, and a transmembranous helix at the C-terminus. In odontoblasts, the proteins are located in Golgi vesicles. However, they display a broader expression pattern, since they are also expressed by nerve fibers in the dental pulp and other tissues (e.g., trachea, brain, kidney), as demonstrated by immunohistochemistry and qPCR, respectively. Their location in odontoblasts suggests a role in collagen and glycosaminoglycan synthesis.
ISSN:0022-0345
1544-0591
DOI:10.1177/154405910808700209