Determination of two HMG-CoA reductase inhibitors, pravastatin and pitavastatin, in plasma samples using liquid chromatography-tandem mass spectrometry for pharmaceutical study

We developed a method for determining pravastatin or pitavastatin, 3‐hydroxy‐3‐methylglutaryl coenzyme A (HMG‐CoA) reductase inhibitors, in plasma using liquid chromatography and tandem mass spectrometry (LC–MS/MS). Pravastatin, pitavastatin and the internal standard fluvastatin were extracted from...

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Published in:Biomedical chromatography 2008-02, Vol.22 (2), p.131-135
Main Authors: Deng, Jian-Wei, Kim, Kwon-Bok, Song, Im-Sook, Shon, Ji-Hong, Zhou, Hong-Hao, Liu, Kwang-Hyeon, Shin, Jae-Gook
Format: Article
Language:English
Subjects:
Chromatography, Liquid - methods
Fatty Acids, Monounsaturated - blood
Fatty Acids, Monounsaturated - chemistry
HMG-CoA
Humans
Hydroxymethylglutaryl-CoA Reductase Inhibitors - blood
Indoles - blood
Indoles - chemistry
LC-MS/MS
pitavastatin
pravastatin
Pravastatin - blood
Quinolines - blood
Tandem Mass Spectrometry - methods
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Summary:We developed a method for determining pravastatin or pitavastatin, 3‐hydroxy‐3‐methylglutaryl coenzyme A (HMG‐CoA) reductase inhibitors, in plasma using liquid chromatography and tandem mass spectrometry (LC–MS/MS). Pravastatin, pitavastatin and the internal standard fluvastatin were extracted from plasma with solid‐phase extraction columns and eluted with methanol. After drying the organic layer, the residue was reconstituted in mobile phase (acetonitrile:water, 90:10, v/v) and injected onto a reversed‐phase C18 column. The isocratic mobile phase was eluted at 0.2 mL/min. The ion transitions recorded in multiple reaction monitoring mode were m/z 423 → 101, 420 → 290 and 410 → 348 for pravastatin, pitavastatin and fluvastatin, respectively. The coefficient of variation of the assay precision was less than 12.4%, the accuracy exceeded 89%. The limit of detection was 1 ng/mL for all analytes. This method was used to measure the plasma concentration of pitavastatin or pravastatin from healthy subjects after a single 4 mg oral dose of pitavastatin or 40 mg oral dose of pravastatin. This is a very simple, sensitive and accurate analytic method to determine the pharmacokinetic profiles of pitavastatin or pravastatiny. Copyright © 2007 John Wiley & Sons, Ltd.
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.905