Fucoidan Inhibits UVB-Induced MMP-1 Expression in Human Skin Fibroblasts

Ultraviolet (UV)B irradiation induces the production of matrix metalloproteinases (MMPs) by activating cellular signaling transduction pathways, which are responsible for the degradation or synthesis inhibition of collagenous extracellular matrix in connective tissues, causing skin photoaging. Using...

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Bibliographic Details
Published in:Biological & Pharmaceutical Bulletin 2008/02/01, Vol.31(2), pp.284-289
Main Authors: Moon, Hee Jung, Lee, Soon Rye, Shim, Sun Nyu, Jeong, Seok Hoon, Stonik, Valentine A., Rasskazov, Valery A., Zvyagintseva, Tatyana, Lee, Yong Hwan
Format: Article
Language:English
Subjects:
Blotting, Western
Cell Line
Cell Survival - drug effects
Extracellular Signal-Regulated MAP Kinases - biosynthesis
Fibroblasts - drug effects
Fibroblasts - enzymology
Fibroblasts - radiation effects
fucoidan
Humans
JNK Mitogen-Activated Protein Kinases - biosynthesis
Matrix Metalloproteinase 1 - biosynthesis
Matrix Metalloproteinase 1 - radiation effects
matrix metalloproteinase-1
Polysaccharides - pharmacology
Radiation-Protective Agents - pharmacology
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - biosynthesis
RNA, Messenger - isolation & purification
Skin - cytology
Skin - enzymology
Skin - radiation effects
Tetrazolium Salts
Thiazoles
ultraviolet B
Ultraviolet Rays
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Summary:Ultraviolet (UV)B irradiation induces the production of matrix metalloproteinases (MMPs) by activating cellular signaling transduction pathways, which are responsible for the degradation or synthesis inhibition of collagenous extracellular matrix in connective tissues, causing skin photoaging. Using the human skin fibroblast (HS68) cell line in the present study, we investigated the inhibitory effects of fucoidan on MMP-1 expression by various in vitro experiments and elucidated the pathways of inhibition. Pretreatment with fucoidan inhibited UVB-induced MMP-1 expression in a dose-dependent manner. Extracellular signal regulated kinase (ERK) activation was markedly inhibited by treatment with fucoidan, though JNK activation was very slightly affected by fucoidan. We also found that fucoidan pretreatment significantly reduced MMP-1 mRNA expression in comparison with UVB irradiation only. In conclusion, our results demonstrate that fucoidan can mainly inhibit UVB-induced MMP-1 expression by inhibiting the ERK pathways. Therefore, fucoidan might be used as a potential agent for the prevention and treatment of skin photoaging.
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.31.284