Inhibition of avian metapneumovirus (AMPV) replication by RNA interference targeting nucleoprotein gene (N) in cultured cells

Avian metapneumovirus (AMPV) is the primary causative agent of severe rhinotracheitis in turkeys. It is associated with swollen head syndrome in chickens and is the source of significant economic losses to animal food production. In this study, we designed specific short interfering RNA (siRNA) targ...

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Bibliographic Details
Published in:Antiviral research 2007-04, Vol.74 (1), p.77-81
Main Authors: Ferreira, Helena Lage, Spilki, Fernando Rosado, Almeida, Renata Servan de, Santos, Márcia M.A.B., Arns, Clarice Weis
Format: Article
Language:English
Subjects:
Animals
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antiviral agents
Avian metapneumovirus
Biological and medical sciences
Cell Line
Chick Embryo
Inhibition
Medical sciences
Metapneumovirus - genetics
Metapneumovirus - growth & development
Paramyxoviridae Infections - virology
Pharmacology. Drug treatments
Real time PCR
RNA Interference
RNA, Small Interfering - chemical synthesis
RNA, Small Interfering - genetics
RNA, Viral - chemical synthesis
RNA, Viral - genetics
Short interfering RNA
Species Specificity
Transfection - methods
Viral Fusion Proteins - genetics
Viral Proteins - genetics
Virus Replication
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Summary:Avian metapneumovirus (AMPV) is the primary causative agent of severe rhinotracheitis in turkeys. It is associated with swollen head syndrome in chickens and is the source of significant economic losses to animal food production. In this study, we designed specific short interfering RNA (siRNA) targeting the AMPV nucleoprotein (N) and fusion (F) genes. Three days post-virus infection, virus titration, real time RT-PCR, and RT-PCR assays were performed to verify the effect of siRNA in AMPV replication. A marked decrease in virus titers from transfected CER cells treated with siRNA/N was observed. Also, the production of N, F, and G mRNAs in AMPV was decreased. Results indicate that N-specific siRNA can inhibit virus replication. In future studies, a combination of siRNAs targeting the RNA polymerase complex may be used as a tool to study AMPV replication and/or antiviral therapy.
ISSN:0166-3542
1872-9096
DOI:10.1016/j.antiviral.2006.12.002