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Identification of Pentatrichomonas hominis in feline fecal samples by polymerase chain reaction assay

Pentatrichomonas hominis is considered to be a commensal protozoan of the vertebrate digestive tract. On the basis of light microscopic examination of feces, some investigators presumptively identified P. hominis as a causative agent of feline diarrhea. However, molecular identification of P. homini...

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Published in:Veterinary parasitology 2007-04, Vol.145 (1), p.11-15
Main Authors: Gookin, Jody L., Stauffer, Stephen H., Levy, Michael G.
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description Pentatrichomonas hominis is considered to be a commensal protozoan of the vertebrate digestive tract. On the basis of light microscopic examination of feces, some investigators presumptively identified P. hominis as a causative agent of feline diarrhea. However, molecular identification of P. hominis infection in the cat has not been reported. Another trichomonad, Tritrichomonas foetus, is recognized as an intestinal pathogen in cats and often presumptively diagnosed on the basis of the presence of trichomonads in diarrheic feces. It is of importance to determine if cats are natural hosts for P. hominis, as the presence of this organism could result in inaccurate assumption of T. foetus infection. In this study, we used a species-specific PCR assay to identify P. hominis 18S rRNA genes in fecal samples collected from a convenience population of cats in which a high prevalence of T. foetus infection had been previously identified (cat show) or suspected (submitted for T. foetus diagnostic testing). The prevalence of T. foetus infection in these samples was 31% and 28.6%, respectively. P. hominis infection was identified by PCR of DNA extracted from feces of five cats (1.9% and 2.1% of fecal samples, respectively). All cats in which P. hominis was identified were also infected with T. foetus. PCR identification of P. hominis infection in the cat should facilitate future studies to determine the pathogenicity of this species and enable differentiation of P. hominis from other known or as-yet unidentified species of trichomonads that may infect cats.
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identifier ISSN: 0304-4017
ispartof Veterinary parasitology, 2007-04, Vol.145 (1), p.11-15
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source ScienceDirect Journals
subjects Animals
Cat Diseases - diagnosis
Cat Diseases - parasitology
cats
Cats - parasitology
Diarrhea
disease detection
disease prevalence
feces
Feces - parasitology
mixed infection
molecular systematics
pathogen identification
Pentatrichomonas hominis
polymerase chain reaction
Polymerase Chain Reaction - methods
Polymerase Chain Reaction - veterinary
protozoal infections
ribosomal DNA
RNA, Protozoan - analysis
RNA, Protozoan - genetics
RNA, Ribosomal, 16S - genetics
RNA, Ribosomal, 18S - genetics
Trichomonadida - genetics
Trichomonadida - isolation & purification
Trichomonas
Trichomonas Infections - diagnosis
Trichomonas Infections - veterinary
trichomoniasis
Tritrichomonas foetus
title Identification of Pentatrichomonas hominis in feline fecal samples by polymerase chain reaction assay
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