Tumour Suppressor Protein (p53), Apoptosis Inhibiting Protein (Bcl-2) and Proliferating Cell Nuclear Antigen (PCNA) Expressions in a Rat Pancreatic Tumour Model

Background: Previous studies have shown that, cultured rat pancreatic carcinoma cells, derived from azaserine-induced acinar tumours, yield tumours with a ductal phenotype. Materials and Methods: In order to find out the molecular characteristics of this tumour model, tumour suppressor protein (p53)...

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Bibliographic Details
Published in:Anticancer research 2007-01, Vol.27 (1A), p.23-26
Main Authors: MAKINENI, Kimmo, LOIMAS, Sami, HAKALA, Tapio, ESKELINEN, Matti
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Disease Models, Animal
Gastroenterology. Liver. Pancreas. Abdomen
Immunohistochemistry
Liver. Biliary tract. Portal circulation. Exocrine pancreas
Male
Medical sciences
Pancreatic Neoplasms - metabolism
Proliferating Cell Nuclear Antigen - biosynthesis
Proto-Oncogene Proteins c-bcl-2 - biosynthesis
Rats
Rats, Inbred Lew
Tumor Suppressor Protein p53 - biosynthesis
Tumors
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Summary:Background: Previous studies have shown that, cultured rat pancreatic carcinoma cells, derived from azaserine-induced acinar tumours, yield tumours with a ductal phenotype. Materials and Methods: In order to find out the molecular characteristics of this tumour model, tumour suppressor protein (p53), apoptosis inhibiting protein (Bcl-2) and proliferating cell nuclear antigen (PCNA) expressions were analysed in rat pancreatic and subcutaneous tumours, as well as in normal rat pancreas. Results: Immunoreactivity for p53 protein was found in 86% of intrapancreatic tumours and in 100% of subcutaneous tumours. The average fraction of positive carcinoma cells was over 50%. Normal rat pancreas showed only slight positive or negative staining for p53. Bcl-2 did not show positive immunoreactivity in rat tumour samples. For PCNA all tumour samples showed positive staining. Also normal pancreas of 6-week-old animals were clearly positive, whereas the samples of the older animals were only slightly positive. Conclusion: Possible mutations in the p53 tumour suppressor gene and a strong expression of PCNA were shown in carcinoma cell line-induced rat pancreatic tumours. These features of the rat pancreatic tumour model resemble human pancreatic carcinoma and may favour the use of this model in pancreatic cancer studies.
ISSN:0250-7005
1791-7530