The detection of serum anti-p53 antibodies from patients with gastric carcinoma in China

• Published in: Cancer detection and prevention 2007, Vol.31 (1), p.45-49
• Main Authors: Qiu, Lin-Lin, MSc, Hua, Pan-Yu, PhD, Ye, Li-Li, MSc, Wang, Yong-Cai, BS, Qiu, Tian, MSc, Bao, Hui-Zheng, PhD, Wang, Li, PhD
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Age; Aged; Anti-p53 antibodies; Antibodies, Neoplasm - blood; Autoantibodies - blood; Binding sites; Biomarkers, Tumor - blood; Blotting, Western; Breast cancer; Cancer; Carcinoembryonic Antigen - blood; Case-Control Studies; China; ELISA; Enzyme-Linked Immunosorbent Assay; Epidemiology; Female; Gastric carcinoma; Hematology, Oncology and Palliative Medicine; Humans; Male; Middle Aged; Neoplasm Staging; Peptide Library; Peptides; Phage-displayed peptide; Proteins; Recombinant wild-type human p53 protein; Stomach Neoplasms - immunology; Stomach Neoplasms - pathology; Tumor Suppressor Protein p53 - immunology; Tumors
• ISSN: 0361-090X; 1877-7821; 1873-443X; 1877-783X
• DOI: 10.1016/j.cdp.2006.12.005

Abstract Background : Gastric carcinoma is one of the most frequently occurring cancers. The aim of this research was to increase the detection efficiency of anti-p53 antibodies in the sera of patients with gastric carcinoma and to improve the diagnosis for patients with gastric carcinoma. Methods : We prepared phage-displayed peptide DO7 and established an enzyme-linked immunosorbent assay method to detect the anti-p53 antibodies. We detected the anti-p53 antibodies of 61 patients with gastric carcinoma using the method and our previous ELISA method assisted by the recombinant wild-type human p53 protein to detect the anti-p53 antibodies. We studied the correlation between the anti-p53 antibodies and the clinicopathological data including sex, age, carcinoembryonic antigen, tumor size, tumor TNM staging, and lymph-node status. Results : The anti-p53 antibodies positive rate for patients with gastric carcinoma was increased (31.1%, 19/61) through the combination of p53-ELISA and phage-ELISA. We found that the positive anti-p53 antibodies correlated significantly with tumor size ( P = 0.047). The combination of the anti-p53 antibodies and carcinoembryonic antigen could improve the diagnosis for patients with gastric carcinoma. Conclusions : This approach indicated an increased anti-p53 antibodies positive rate for patients with gastric carcinoma and provided a useful marker for clinical diagnosis for patients with gastric carcinoma.