Genetic and antigenic relatedness of H3 subtype influenza A viruses isolated from avian and mammalian species

• Published in: Vaccine 2008-02, Vol.26 (7), p.966-977
• Main Authors: Yassine, H.M, Lee, C.W, Suarez, D.L, Saif, Y.M
• Format: Article
• Language: English
• Subjects: Allergy and Immunology; Amino Acid Sequence; Animals; Applied microbiology; Aquatic birds; Biological and medical sciences; Comparative studies; Ducks; Ducks - virology; Fundamental and applied biological sciences. Psychology; H3N2 triple reassortants; Hemagglutinin Glycoproteins, Influenza Virus - chemistry; Hemagglutinin Glycoproteins, Influenza Virus - genetics; Hemagglutinin Glycoproteins, Influenza Virus - immunology; Hogs; Humans; Immunization; Infections; Influenza; Influenza A virus - classification; Influenza A virus - genetics; Influenza A virus - immunology; Influenza A virus - isolation & purification; Influenza A Virus, H3N2 Subtype - classification; Influenza A Virus, H3N2 Subtype - genetics; Influenza A Virus, H3N2 Subtype - immunology; Influenza A Virus, H3N2 Subtype - isolation & purification; Influenza in Birds - immunology; Influenza in Birds - virology; Influenza, Human - immunology; Influenza, Human - virology; Microbiology; Models, Molecular; Orthomyxoviridae Infections - immunology; Orthomyxoviridae Infections - virology; Phylogeny; Reassortant Viruses - genetics; Reassortant Viruses - immunology; Reassortant Viruses - isolation & purification; Sequence Alignment; Swine; Swine - virology; Swine Diseases - virology; Turkeys; Turkeys - virology; Vaccine; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Viruses
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2007.11.094

Abstract In 2004, we isolated triple reassortant H3N2 influenza viruses from turkey breeder hens in Ohio and Illinois. The Illinois flock was vaccinated twice with an inactivated H3N2 vaccine containing a swine origin virus before the outbreak. Additionally, a commercial inactivated vaccine containing an H3N4 virus of duck origin is being used in some turkey breeders. This prompted us to initiate a comparative study on the antigenic and genetic relatedness of various H3 subtype influenza viruses isolated from turkeys, ducks, pigs and humans. The antigenic relatedness between the different viruses was evaluated with the Archetti and Horsfall formula, while nucleotide genetic similarities were calculated using pairwise alignments. Results obtained indicated a high degree of antigenic (>90%) and genetic (>99%) similarities among the turkey-origin H3N2 viruses. However, the turkey viruses were antigenically distantly related to the swine-origin vaccine virus (