Potato glycoalkaloids in soil-optimising liquid chromatography–time-of-flight mass spectrometry for quantitative studies

• Published in: Journal of Chromatography A 2008-02, Vol.1182 (1), p.65-71
• Main Authors: Jensen, Pia H., Juhler, René K., Nielsen, Nikoline J., Hansen, Thomas H., Strobel, Bjarne W., Jacobsen, Ole S., Nielsen, John, Hansen, Hans Christian B.
• Format: Article
• Language: English
• Subjects: agricultural soils; Agronomy. Soil science and plant productions; Analysis methods; Analytical chemistry; Applied sciences; Biological and medical sciences; chemical residues; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, Liquid - methods; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; glycoalkaloids; LC–MS; liquid chromatography; mass spectrometry; metabolites; Molecular Structure; Natural toxins; Other chromatographic methods; Pollution; potatoes; Reproducibility of Results; Soil - analysis; Soil and sediments pollution; Soil and water pollution; Soil extract; Soil science; Solanaceous Alkaloids - analysis; Solanaceous Alkaloids - chemistry; Solanidine; Solanine - analogs & derivatives; Solanine - analysis; Solanine - chemistry; Solanum tuberosum; Solanum tuberosum - chemistry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; time-of-flight mass spectrometry; α-Chaconine; α-Solanine
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2007.12.049

Potato glycoalkaloids are produced in high amounts in potato fields during the growth season and losses to soil potentially impact shallow groundwater and via tiles to fresh water ecosystems. A quantitative liquid chromatography–electrospray ionization time-of-flight mass spectrometry (LC–ESI-TOF-MS) method for determination and quantification of potato glycoalkaloids and their metabolites in aqueous soil extracts was developed. The LC–ESI-TOF-MS method had linearities up to 2000 μg/L for α-solanine and α-chaconine and up to 760 μg/L for solanidine. No matrix effect was observed, and the detection limits found were in the range 2.2–4.7 μg/L. The method enabled quantification of the potato glycoalkaloids in environmental samples.