Cytotoxic Activity and Absence of Tumor Growth Stimulation of Standardized Mistletoe Extracts in Human Tumor Models In Vitro

• Published in: Anticancer research 2007-01, Vol.27 (1A), p.223-233
• Main Authors: KELTER, Gerhard, SCHIERHOLZ, Jörg M, FISCHER, Imma U, FIEBIG, Heinz-Herbert
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Cell Growth Processes - drug effects; Cell Line, Tumor; Colonic Neoplasms - drug therapy; Colonic Neoplasms - pathology; Doxorubicin - pharmacology; Humans; Interleukin-6 - pharmacology; Medical sciences; Melanoma - drug therapy; Melanoma - pathology; Mistletoe - chemistry; Neoplasms - drug therapy; Neoplasms - pathology; Plant Extracts - pharmacology; Plant Preparations - pharmacology; Plant Proteins - pharmacology; Ribosome Inactivating Proteins, Type 2; Sarcoma - drug therapy; Sarcoma - pathology; Toxins, Biological - pharmacology; Tumors
• ISSN: 0250-7005; 1791-7530

Mistletoe extracts are widely used in complementary and alternative cancer therapy in Europe. The extracts possess cytotoxic, as well as immunostimulatory effects. However, some investigators have suggested that low doses of mistletoe extracts could also induce tumor growth. The mistletoe extracts Helixor A, Helixor M and Helixor P were investigated for growth inhibitory and stimulatory effects in a panel of 38 human tumor cell lines in vitro. Mistletoe lectin I (ML-1), adriamycin and interleukin-6 (IL-6) were used as reference compounds. All three mistletoe preparations showed cytotoxic activity [T/C (Test/Control) 125%) by the mistletoe extracts or ML-1, apart from two exceptions in the colon carcinoma cell line HCC-2998, in which Helixor M and ML-1 showed a marginal stimulation (T/C 128% and 131%, respectively) at one concentration only. Further investigations into the latter effect of Helixor M and ML-1 in the HCC-2998 line using five different proliferation assays, modified cell culture conditions and the identical production charge of mistletoe extract, as well as a new one, did not confirm the previous observation. It was concluded that the marginal stimulation found in the earlier experiments was a statistical coincidence. Helixor mistletoe preparations and ML-1 have cytotoxic activity and do not stimulate tumor cell proliferation in vitro which is in accordance with previous scientifically based observations on aqueous mistletoe extracts.