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Production of soft rot resistant calla lily by expressing a ferredoxin-like protein gene (pflp) in transgenic plants
An efficient protocol for the Agrobacterium tumefaciens-mediated transformation of calla lily (Zantedeschia elliottiana (W. Wats.) Engl. cultivar 'Florex Gold') is described. Shoot basal discs were co-cultivated with A. tumefaciens C58C1 carrying a plasmid containing neomycin phosphotransf...
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| Published in: | Plant cell reports 2007-04, Vol.26 (4), p.449-457 |
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| container_title | Plant cell reports |
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| creator | Yip, Mei-Kuen Huang, Hsiang-En Ger, Mang-Jye Chiu, Shih-Hua Tsai, Yuh-Chih Lin, Chin-I Feng, Teng-Yung |
| description | An efficient protocol for the Agrobacterium tumefaciens-mediated transformation of calla lily (Zantedeschia elliottiana (W. Wats.) Engl. cultivar 'Florex Gold') is described. Shoot basal discs were co-cultivated with A. tumefaciens C58C1 carrying a plasmid containing neomycin phosphotransferase (nptII) and plant ferredoxin-like protein (pflp) genes. After Agrobacterium co-cultivation, the shoot basal discs were exposed to 100 mg l-¹ kanamycin for selection. Twenty-eight out of 260 discs (10.8%) were found to have survived and produced shoot clusters. Twenty-six of these were confirmed to contain the pflp transgene by PCR, ending up in 10% transformation efficiency. The disease resistance investigation revealed that 18 transgenic plants exhibited resistance to soft rot disease caused by Erwinia carotovora subsp. carotovora. The presence of pflp gene was demonstrated by PCR, and its accumulation and activity was confirmed by Western blot and disease resistance assay. This was the first report to show the successful transformation and resistance to a bacterial pathogen in Zantedeschia. The protocol is useful for the quality improvement of calla lily through genetic transformation. |
| doi_str_mv | 10.1007/s00299-006-0246-y |
| format | article |
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Wats.) Engl. cultivar 'Florex Gold') is described. Shoot basal discs were co-cultivated with A. tumefaciens C58C1 carrying a plasmid containing neomycin phosphotransferase (nptII) and plant ferredoxin-like protein (pflp) genes. After Agrobacterium co-cultivation, the shoot basal discs were exposed to 100 mg l-¹ kanamycin for selection. Twenty-eight out of 260 discs (10.8%) were found to have survived and produced shoot clusters. Twenty-six of these were confirmed to contain the pflp transgene by PCR, ending up in 10% transformation efficiency. The disease resistance investigation revealed that 18 transgenic plants exhibited resistance to soft rot disease caused by Erwinia carotovora subsp. carotovora. The presence of pflp gene was demonstrated by PCR, and its accumulation and activity was confirmed by Western blot and disease resistance assay. This was the first report to show the successful transformation and resistance to a bacterial pathogen in Zantedeschia. The protocol is useful for the quality improvement of calla lily through genetic transformation.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s00299-006-0246-y</identifier><identifier>PMID: 17033825</identifier><identifier>CODEN: PCRPD8</identifier><language>eng</language><publisher>Berlin: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Agrobacterium ; Agrobacterium tumefaciens - genetics ; Biological and medical sciences ; Biotechnology ; Blotting, Southern ; Blotting, Western ; Calla ; Cultivars ; Disease resistance ; DNA, Plant - analysis ; DNA, Plant - genetics ; Erwinia carotovora ; Ferredoxin-like protein ; Ferredoxins - genetics ; Ferredoxins - metabolism ; Flowers & plants ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic technics ; Methods. Procedures. Technologies ; Pectobacterium carotovorum - growth & development ; Plant Diseases - genetics ; Plant Diseases - microbiology ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plants, Genetically Modified ; Polymerase Chain Reaction ; Soft rot disease resistance ; Transformation, Genetic ; Transgenic ; Transgenic animals and transgenic plants ; Transgenic plants ; Zantedeschia ; Zantedeschia - genetics ; Zantedeschia - metabolism ; Zantedeschia - microbiology ; Zantedeschia elliottiana</subject><ispartof>Plant cell reports, 2007-04, Vol.26 (4), p.449-457</ispartof><rights>2007 INIST-CNRS</rights><rights>Springer-Verlag 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c411t-f4754ba2908dabcf22de448f4ae993dd8549183e2fcc7e100dee94bcd3238563</citedby><cites>FETCH-LOGICAL-c411t-f4754ba2908dabcf22de448f4ae993dd8549183e2fcc7e100dee94bcd3238563</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18671014$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17033825$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yip, Mei-Kuen</creatorcontrib><creatorcontrib>Huang, Hsiang-En</creatorcontrib><creatorcontrib>Ger, Mang-Jye</creatorcontrib><creatorcontrib>Chiu, Shih-Hua</creatorcontrib><creatorcontrib>Tsai, Yuh-Chih</creatorcontrib><creatorcontrib>Lin, Chin-I</creatorcontrib><creatorcontrib>Feng, Teng-Yung</creatorcontrib><title>Production of soft rot resistant calla lily by expressing a ferredoxin-like protein gene (pflp) in transgenic plants</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><description>An efficient protocol for the Agrobacterium tumefaciens-mediated transformation of calla lily (Zantedeschia elliottiana (W. Wats.) Engl. cultivar 'Florex Gold') is described. Shoot basal discs were co-cultivated with A. tumefaciens C58C1 carrying a plasmid containing neomycin phosphotransferase (nptII) and plant ferredoxin-like protein (pflp) genes. After Agrobacterium co-cultivation, the shoot basal discs were exposed to 100 mg l-¹ kanamycin for selection. Twenty-eight out of 260 discs (10.8%) were found to have survived and produced shoot clusters. Twenty-six of these were confirmed to contain the pflp transgene by PCR, ending up in 10% transformation efficiency. The disease resistance investigation revealed that 18 transgenic plants exhibited resistance to soft rot disease caused by Erwinia carotovora subsp. carotovora. The presence of pflp gene was demonstrated by PCR, and its accumulation and activity was confirmed by Western blot and disease resistance assay. This was the first report to show the successful transformation and resistance to a bacterial pathogen in Zantedeschia. The protocol is useful for the quality improvement of calla lily through genetic transformation.</description><subject>Agrobacterium</subject><subject>Agrobacterium tumefaciens - genetics</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Blotting, Southern</subject><subject>Blotting, Western</subject><subject>Calla</subject><subject>Cultivars</subject><subject>Disease resistance</subject><subject>DNA, Plant - analysis</subject><subject>DNA, Plant - genetics</subject><subject>Erwinia carotovora</subject><subject>Ferredoxin-like protein</subject><subject>Ferredoxins - genetics</subject><subject>Ferredoxins - metabolism</subject><subject>Flowers & plants</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Methods. Procedures. Technologies</subject><subject>Pectobacterium carotovorum - growth & development</subject><subject>Plant Diseases - genetics</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plants, Genetically Modified</subject><subject>Polymerase Chain Reaction</subject><subject>Soft rot disease resistance</subject><subject>Transformation, Genetic</subject><subject>Transgenic</subject><subject>Transgenic animals and transgenic plants</subject><subject>Transgenic plants</subject><subject>Zantedeschia</subject><subject>Zantedeschia - genetics</subject><subject>Zantedeschia - metabolism</subject><subject>Zantedeschia - microbiology</subject><subject>Zantedeschia elliottiana</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqFkUuLFDEQgIMo7jj6A7xoEBQ9tFYe3UkfZfEFCwqu4C1k0pUha093b9IN2__eGmZgwYuHEJL6qiqpj7HnAt4LAPOhAMi2rQCaCqRuqvUB2witZCVB_X7INmCkqIwR-oI9KeUGgIKmecwuhAGlrKw3bP6Rx24JcxoHPkZexjjzPNLCksrsh5kH3_ee96lf-W7leDdRqKRhzz2PmDN2410aqj79QT5RJqaB73FA_naK_fSO03HOfih0lwKfeipZnrJH0fcFn533Lbv-_On68mt19f3Lt8uPV1XQQsxV1KbWOy9bsJ3fhShlh1rbqD22reo6W-tWWIUyhmCQJtIhtnoXOiWVrRu1ZW9OZeldtwuW2R1SCUjfGXBcijMgGxog_BeUYEFBIwl89Q94My55oD84C6aWphXHtuIEhTyWkjG6KaeDz6sT4I7e3MmbI2_u6M2tlPPiXHjZHbC7zziLIuD1GfCFjEQaaUjlnrONEUe9W_byxEU_Or_PxPz6KUEo6lsbsEL9Bb8Jqpk</recordid><startdate>20070401</startdate><enddate>20070401</enddate><creator>Yip, Mei-Kuen</creator><creator>Huang, Hsiang-En</creator><creator>Ger, Mang-Jye</creator><creator>Chiu, Shih-Hua</creator><creator>Tsai, Yuh-Chih</creator><creator>Lin, Chin-I</creator><creator>Feng, Teng-Yung</creator><general>Berlin/Heidelberg : Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>20070401</creationdate><title>Production of soft rot resistant calla lily by expressing a ferredoxin-like protein gene (pflp) in transgenic plants</title><author>Yip, Mei-Kuen ; Huang, Hsiang-En ; Ger, Mang-Jye ; Chiu, Shih-Hua ; Tsai, Yuh-Chih ; Lin, Chin-I ; Feng, Teng-Yung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c411t-f4754ba2908dabcf22de448f4ae993dd8549183e2fcc7e100dee94bcd3238563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Agrobacterium</topic><topic>Agrobacterium tumefaciens - genetics</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Blotting, Southern</topic><topic>Blotting, Western</topic><topic>Calla</topic><topic>Cultivars</topic><topic>Disease resistance</topic><topic>DNA, Plant - analysis</topic><topic>DNA, Plant - genetics</topic><topic>Erwinia carotovora</topic><topic>Ferredoxin-like protein</topic><topic>Ferredoxins - genetics</topic><topic>Ferredoxins - metabolism</topic><topic>Flowers & plants</topic><topic>Fundamental and applied biological sciences. 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Academic</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yip, Mei-Kuen</au><au>Huang, Hsiang-En</au><au>Ger, Mang-Jye</au><au>Chiu, Shih-Hua</au><au>Tsai, Yuh-Chih</au><au>Lin, Chin-I</au><au>Feng, Teng-Yung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production of soft rot resistant calla lily by expressing a ferredoxin-like protein gene (pflp) in transgenic plants</atitle><jtitle>Plant cell reports</jtitle><addtitle>Plant Cell Rep</addtitle><date>2007-04-01</date><risdate>2007</risdate><volume>26</volume><issue>4</issue><spage>449</spage><epage>457</epage><pages>449-457</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><coden>PCRPD8</coden><abstract>An efficient protocol for the Agrobacterium tumefaciens-mediated transformation of calla lily (Zantedeschia elliottiana (W. Wats.) Engl. cultivar 'Florex Gold') is described. Shoot basal discs were co-cultivated with A. tumefaciens C58C1 carrying a plasmid containing neomycin phosphotransferase (nptII) and plant ferredoxin-like protein (pflp) genes. After Agrobacterium co-cultivation, the shoot basal discs were exposed to 100 mg l-¹ kanamycin for selection. Twenty-eight out of 260 discs (10.8%) were found to have survived and produced shoot clusters. Twenty-six of these were confirmed to contain the pflp transgene by PCR, ending up in 10% transformation efficiency. The disease resistance investigation revealed that 18 transgenic plants exhibited resistance to soft rot disease caused by Erwinia carotovora subsp. carotovora. The presence of pflp gene was demonstrated by PCR, and its accumulation and activity was confirmed by Western blot and disease resistance assay. This was the first report to show the successful transformation and resistance to a bacterial pathogen in Zantedeschia. The protocol is useful for the quality improvement of calla lily through genetic transformation.</abstract><cop>Berlin</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>17033825</pmid><doi>10.1007/s00299-006-0246-y</doi><tpages>9</tpages></addata></record> |
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| ispartof | Plant cell reports, 2007-04, Vol.26 (4), p.449-457 |
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| language | eng |
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| source | Springer Nature |
| subjects | Agrobacterium Agrobacterium tumefaciens - genetics Biological and medical sciences Biotechnology Blotting, Southern Blotting, Western Calla Cultivars Disease resistance DNA, Plant - analysis DNA, Plant - genetics Erwinia carotovora Ferredoxin-like protein Ferredoxins - genetics Ferredoxins - metabolism Flowers & plants Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Methods. Procedures. Technologies Pectobacterium carotovorum - growth & development Plant Diseases - genetics Plant Diseases - microbiology Plant Proteins - genetics Plant Proteins - metabolism Plants, Genetically Modified Polymerase Chain Reaction Soft rot disease resistance Transformation, Genetic Transgenic Transgenic animals and transgenic plants Transgenic plants Zantedeschia Zantedeschia - genetics Zantedeschia - metabolism Zantedeschia - microbiology Zantedeschia elliottiana |
| title | Production of soft rot resistant calla lily by expressing a ferredoxin-like protein gene (pflp) in transgenic plants |
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