Disruption of srtA gene in Streptococcus suis results in decreased interactions with endothelial cells and extracellular matrix proteins

• Published in: Veterinary microbiology 2008-03, Vol.127 (3), p.417-424
• Main Authors: Vanier, G., Sekizaki, T., Domínguez-Punaro, M.C., Esgleas, M., Osaki, M., Takamatsu, D., Segura, M., Gottschalk, M.
• Format: Article
• Language: English
• Subjects: adhesion; Aminoacyltransferases - genetics; animal models; animal proteins; Animals; Bacterial Adhesion - physiology; bacterial infections; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacteriology; Biological and medical sciences; Cell Line; Cells, Cultured; collagen; Collagen - metabolism; Cysteine Endopeptidases - genetics; Disease Models, Animal; Endothelial cells; Endothelial Cells - microbiology; enzymes; extracellular matrix; Extracellular matrix proteins; Extracellular Matrix Proteins - metabolism; Female; fibronectins; Fibronectins - metabolism; Fundamental and applied biological sciences. Psychology; genes; Humans; LPXTG motif; Mice; Mice, Inbred Strains; microbial genetics; Microbiology; Miscellaneous; mutants; Mutation; Porcine; Protein Binding; Sortase A; srtA gene; Streptococcal Infections - microbiology; Streptococcus suis; Streptococcus suis - genetics; Streptococcus suis - pathogenicity; Streptococcus suis - physiology; Swine; virulence; Virulence - physiology
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2007.08.032

Streptococcus suis, a major pathogen of swine, is an emerging zoonotic agent which causes meningitis and septic shock. In this study, we investigated the ability of S. suis mutant strain (SRTΔA) lacking the sortase A gene ( srtA) to interact with host cells and extracellular matrix (ECM) proteins, as well as its virulence in a mouse infection model. We demonstrated that mutant SRTΔA had reduced capacity to adhere to and invade porcine brain microvascular endothelial cells compared to the wild-type strain. In addition, mutant SRTΔA also showed significantly less adherence to plasma fibronectin, cellular fibronectin and collagen type I. However, disruption of srtA had little effect on the virulence of S. suis in a mouse intraperitoneal model of infection. These results indicate that surface proteins anchored by sortase A are required for a normal level of bacterial binding. However, other factors may also be important for S. suis virulence and interaction with host tissues.