Simultaneous high-performance liquid chromatographic determination of Cedrus deodara active constituents and their pharmacokinetic profile in mice

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2008-02, Vol.862 (1), p.237-241
• Main Authors: Sachin, B.S., Koul, M., Zutshi, A., Singh, S.K., Tikoo, A.K., Tikoo, M.K., Saxena, A.K., Sharma, S.C., Johri, R.K.
• Format: Article
• Language: English
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Cedrus - chemistry; Cedrus deodara; Chromatography, High Pressure Liquid - methods; Dibenzylbutyrolactol; Fundamental and applied biological sciences. Psychology; General pharmacology; Matairesinol; Medical sciences; Mice; Pharmacokinetics; Pharmacology. Drug treatments; Plant Extracts - chemistry; Plant Extracts - pharmacokinetics; Reference Standards; Reproducibility of Results; Sensitivity and Specificity; Wikstromol
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2007.12.015

A specific and sensitive high-performance liquid chromatographic (HPLC) method with photodiode-array (PDA) ultraviolet detection was developed for the simultaneous determination of three bioactive constituents of Cedrus deodara namely wikstromol, matairesinol and dibenzylbutyrolactol in mouse plasma. In solid-phase extraction (SPE) these constituents were successfully separated using a C 18 column by isocratic elution using acetonitrile:water containing hexanesulphonic acid, 32:68 (v/v). The flow rate was set at 1 ml/min and detector wavelength at 225 nm. Good linearity ( r 2 > 0.999) was observed over the studied range of 0.015–5.0 μg/ml for wikstromol and 0.030–5.0 μg/ml for matairesinol and dibenzylbutyrolactol. The CV values of intra-day precision for wikstromol, matairesinol and dibenzylbutyrolactol were in between 1.8–6.9, 1.7–4.9 and 1.6–4.2% and values of inter-day precision were in between 10.4–12.2, 9.7–11 and 10–11.2%, respectively. The extraction recoveries at low to high concentration were greater than 98, 83 and 87% for each analyte, respectively. The LOQ for wikstromol was 0.015 μg/ml and for both matairesinol and dibenzylbutyrolactol it was 0.030 μg/ml. The developed method was used to determine the pharmacokinetics of the three analytes in mice after intraperitoneal administration of CD-3.