Trypanosoma evansi: Molecular homogeneity as inferred by phenetical analysis of ribosomal internal transcribed spacers DNA of an eclectic parasite

• Published in: Experimental parasitology 2008-03, Vol.118 (3), p.402-407
• Main Authors: de Oliveira Lima, Aneska Norek, Santos, Simone da Silva, Herrera, Heitor Miraglia, Gama, Carla, Cupolillo, Elisa, Jansen, Ana Maria, Fernandes, Octávio
• Format: Article
• Language: English
• Subjects: Animals; arbitrary primers; Biological and medical sciences; Brazil; Camelus; Cattle; China; deoxyribonucleotide triphosphates; DNA, Protozoan - chemistry; DNA, Ribosomal Spacer - chemistry; dNTPs; Dogs; Ethiopia; Fundamental and applied biological sciences. Psychology; genetic similarity; genetic variation; geographical variation; Horses; hosts; internal transcribed spacer; internal transcribed spacers; ITS; kDNA; kinetoplast DNA; Life cycle. Host-agent relationship. Pathogenesis; nontranscribed spacers; NTS; PCR; Phenetical analysis; Phylogeny; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Procyonidae; Protozoa; random amplification of polymorphic; RAPD; rDNA; restriction fragment length polymorphism; RFLP; ribosomal DNA; Rodentia; Sheep; strain differences; Trypanosoma - classification; Trypanosoma - genetics; Trypanosoma brucei brucei - classification; Trypanosoma brucei brucei - genetics; Trypanosoma evansi; Uganda
• ISSN: 0014-4894; 1090-2449
• DOI: 10.1016/j.exppara.2007.10.003

The protozoan Trypanosoma evansi is described as presenting high morphological and genetic similarities among the isolates despite its biological heterogeneity and wide geographical distribution. PCR amplification of the internal transcribed spacers of the ribosomal gene in combination with the coding region of the 5.8S ribosomal subunit further submitted to restriction enzymes digestion were carried out in DNAs extracted from 41 T. evansi strains isolated from horses, dogs, coatis and capybaras from two distinct regions of the Brazilian Pantanal. We also used one T. evansi isolate from Africa, one from Asia and one isolate of T. b. brucei from Africa. Analysis of the RFLP profiles yielded a unique “riboprinting” that does not vary intraspecifically. These results provide insights on the ribosomal gene organization of T. evansi and showed that ITS analysis by RFLP show high genetic similarity of this locus among isolates of this protozoan parasite.