LC MALDI-TOF MS/MS and LC ESI FTMS analyses of HLA-B27 associated peptides isolated from peripheral blood cells

• Published in: Immunology letters 2008-02, Vol.116 (1), p.79-85
• Main Authors: Stodůlková, Eva, Novák, Petr, Deininger, Sören-Oliver, Man, Petr, Čapková, Jana, Kavan, Daniel, Ivašková, Eva, Flieger, Miroslav
• Format: Article
• Language: English
• Subjects: Adult; Alcohol Oxidoreductases; Allergy and Immunology; Autoimmunity - genetics; Blood Cells - immunology; Blood Cells - metabolism; Databases, Protein; Endopeptidases - metabolism; Genetic Predisposition to Disease; GTP-Binding Protein alpha Subunits, Gs - blood; GTP-Binding Protein alpha Subunits, Gs - genetics; GTP-Binding Protein alpha Subunits, Gs - immunology; Healthy blood donors; Histones - genetics; Histones - metabolism; HLA-B27; HLA-B27 Antigen - analysis; HLA-B27 Antigen - immunology; HLA-B27 Antigen - isolation & purification; HSC70 Heat-Shock Proteins - blood; HSC70 Heat-Shock Proteins - genetics; HSC70 Heat-Shock Proteins - immunology; Humans; LC ESI FTMS; MALDI-TOF MS/MS; Male; Peptide Mapping; Peptides - analysis; Peptides - immunology; Peptides - isolation & purification; Protein Interaction Domains and Motifs; Sequence Alignment; Software; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spondylitis, Ankylosing - genetics; Spondylitis, Ankylosing - metabolism
• ISSN: 0165-2478; 1879-0542
• DOI: 10.1016/j.imlet.2007.11.011

Abstract Peptides eluted from peripheral blood cells of HLA-B*2705 healthy donor were analyzed by LC MALDI MS/MS and LC ESI FTMS techniques. The sequences of 92 peptide ligands identified from one healthy blood donor by LC MALDI-TOF MS/MS were compared with those previously published from in vitro long-term cell cultures available in SYFPEITHI database and splenocytes. It was found that 18 sequences confirmed within 1 ppm mass error by LC ESI FTMS were already described and 3 of them matched with those previously reported from HLA-B*2705 splenocytes. Another 38 sequences validated within the same mass error were not found in SYFPEITHI database and are identified here for the first time. Finally, 36 sequences (5 sequences already published in SYFPEITHI database) were evaluated by LC MALDI-TOF MS/MS but no matches in the list of monoisotopic masses obtained from LC ESI FTMS were found.