Development of an optical RNA-based aptasensor for C-reactive protein

The development of a RNA-aptamer-based optical biosensor (aptasensor) for C-reactive protein (CRP) is reported. CRP is an important clinical biomarker; it was the first acute-phase protein to be discovered (1930) and is a sensitive systemic marker of inflammation and tissue damage. It has also a pro...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2008-02, Vol.390 (4), p.1077-1086
Main Authors: Bini, A, Centi, S, Tombelli, S, Minunni, M, Mascini, M
Format: Article
Language:English
Subjects:
Analytical Chemistry
Aptamer
Aptamers, Nucleotide - chemistry
Base Sequence
Biochemistry
C-reactive protein
C-Reactive Protein - chemistry
Calcium - chemistry
Calibration
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Food Science
Laboratory Medicine
Monitoring/Environmental Analysis
Nucleic Acid Conformation
Optics and Photonics
Original Paper
surface plasmon resonance
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Summary:The development of a RNA-aptamer-based optical biosensor (aptasensor) for C-reactive protein (CRP) is reported. CRP is an important clinical biomarker; it was the first acute-phase protein to be discovered (1930) and is a sensitive systemic marker of inflammation and tissue damage. It has also a prognostic value for patients with acute coronary syndrome. The average concentration of CRP in serum is 0.8 ppm and it increases in response to a variety of inflammatory stimuli, such as trauma, tissue necrosis, infection and myocardial infarction. The interaction between the 44-base RNA aptamer and the target analyte CRP is studied. In particular, the influence of the aptamer immobilization procedure (chemistry, length, concentration), as well as the binding conditions, i.e., the influence on the binding of different buffers, the presence of Ca²⁺ ion and the specificity (against human serum albumin) have been evaluated. Using the best working conditions, we achieved a detection limit of 0.005 ppm, with good selectivity towards human serum albumin. Some preliminary experiments in serum are reported. [graphic removed]
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-007-1736-7